Cloning and Mapping

DNA Nicking

Hydrolyzing the phosphodiester bond of a single strand of the double helix is known as nicking. Creating nicks in DNA is required for nick translation, a technique used to tag a strand of DNA. In nick translation, labeled deoxynucleotide triphosphates (dNTPs) are incorporated into a single strand of DNA by DNA Polymerase I. These labeled dNTPs are then ligated with the existing strand. These labeled fragments can then be used as probes in fluorescent in situ hybridization.

Featured Products

FAQs for DNA Nicking

Protocols for DNA Nicking

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.