Cloning and Mapping
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  • Cloning & Mapping

    Modern-day molecular biology relies on modular manipulations of an organism’s genome to study the behaviors of individual genes. Owing to the stereotyped organization of DNA across species, it is possible to cut-and-paste portions of one organism’s genome into the machinery and language of another organism to exploit improved expression. Generally, cloning refers to the replication of DNA by an organism other than the organism-of-origin. Cloning has become the cornerstone technique of molecular biology and has broad applications. These include the isolation of single genes, the preparation of modified versions of genes and overexpression studies, whether for phenotype analysis or protein production. In contrast to PCR, where a segment of DNA is copied in a cell-free reaction, molecular cloning refers to the use of a living organism, typically bacteria or yeast, to carry out the replication reaction.

    1. What are Restriction Enzymes?

      Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.

    2. The Interaction of Restriction Enzymes and DNA

      Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data.

    3. Discovering New Restriction Enzymes

      Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

    Cloning & Mapping includes these areas of focus:

    Site Directed Mutagenesis
    DNA Analysis
    DNA Isolation
    Synthetic Biology
    Gibson Assembly®
    DNA Ligation
    DNA End Treatment
    DNA Phosphorylation
    DNA Blunting
    DNA Dephosphorylation
    DNA Digestion
    Restriction Enzyme Digestion
    DNA Nicking

    FAQs for Cloning & Mapping

    Protocols for Cloning & Mapping