Gaussia Luciferase

Gaussia Luciferase (GLuc) is a recently discovered, naturally secreted protein by the copepod, Gaussia princeps (1). At 19kD, it is the smallest known luciferase and its catalytic properties make it one of the “brightest” known (2). Additionally, multiple disulfide bonds contribute to the protein stability at elevated temperatures. These properties make it an ideal reporter gene, either as a standalone expression monitor or as a fusion partner with other proteins (3). The first report of in vivo imaging using Gluc demonstrated sensitivity that is several orders of magnitude higher than the first generation luciferases, Firefly Luciferase (Fluc) and Renilla (4). In addition to mammalian cells, the secretion signal of GLuc is functional in all eukaryotic cells, including algae and nematodes. Scientists at NEB have used Gaussia luciferase to develop siRNA potency and miRNA expression assays (5,6). The GLuc reporter is increasingly being used to monitor the cellular secretory pathway and ER stress, to assay for viral infectivity and to study chemokine receptor activation (3,7-10).

Activity of Gaussia (GLuc) and Cypridina (CLuc) Luciferases Expressed in CHO Cells.

Activity was determined from the conditional media or cell lysate.

Extreme Sensitivity of the Gaussia Luciferase Reporter.

HeLa cells were transfected with 1 µg of pCMV-GLuc or pCMV-Renilla, and activity was measured from the cell supernatant (S) or cell lysate (L). Note that although Gaussia Luciferase is a secreted reporter, there is sufficient measurable activity inside the cell. Renilla is only expressed intracellularly.

References

  1. Balou, B., Szent-Gyorgyi, C. and Finley, G (2000) Abstract from 11th Int. Symp. on Biolumin. & Chemilumin. Asilomar, CA. 
  2. Goerke, A., Loening, A., Gambhir, S. and Swartz, J. (2008) Metabolic Engineering, 10, 187–200. PMID: 18555198
  3. Hulleman JD et al.(2011) Mol. Biol. Cell December 15, 2011 vol. 22 no. 244765-4775. PMID: 22031286 
  4. Tannous, B.A. et al (2005) Mol. Ther. 11, 435-443. PMID: 15727940
  5. Morlighem, J.E., Petit, C and Tzertzinis, G. (2007) Biotechniques, 42, 599-606. PMID: 17515198
  6. Shea C. and Tzertzinis G. unpublished observations (2007). 
  7. Badr, et al. (2007) PloS ONE, 2, e571. PMID: 17593970
  8. Hewett, J.W., et al. (2007) Proc. Natl. Acad. Sci, USA, 104, 7271-7276. PMID: 17428918
  9. Capul, A. and de la Torre, J.C. (2008) Virology 382, 107-114. PMID: 18929379
  10. Luker, K., Gupta, M. and Luker G. (2009) Biotechniques, 47, 625-632. PMID: 19594447

Featured Products

Protocols for Gaussia Luciferase

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Advantages

  • Naturally Secreted – Amenable to live cell assays
  • Sensitivity – Brightest luciferases available; enables single cell applications
  • Stability – Samples can be stored for several days with no loss of activity
  • Easy-to-use – Cell lysis not necessary
  • Non-destructive – Living cells can be used in downstream assays
  • Flexible – Activity can also be measured in cell lysates

Applications

  • Transfection optimization studies
  • Promoter/enhancer assays
  • High throughput assays
  • Multiplex assays
  • Multiple assays with other reporters
  • Secretory pathway reporter assays
  • Signal transduction
  • siRNA potency screening
  • Time course studies
  • Single cell assays, including stem cells and primary cells
  • Live cell assays
  • Assays in difficult to transfect cells

NEB vs. Other Commercially Available Reporter Systems

Features Gaussia Cypridina Renilla Firefly Metridia
Transcriptional
Reporter
Extreme Sensitivity      
Secreted    
Stable      
Does not require and is
not affected by ATP
 

Comparison of the features of commercially available reporter systems highlights the advantages of using Gaussia or Cypridina Luciferase from NEB.

Reporter System Selection Chart

NEB offer a variety of products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter assays.