Cellular Analysis
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  • CLIP Surface

    Membrane proteins are challenging to study given their hydrophobic nature, generally low native abundance and intrinsic instability(1,2).  Regardless, half of all protein drug targets are membrane proteins.  For imaging, most fluorescent proteins (i.e. GFP) cannot specifically visualize cell surface subpopulations.  However, CLIP-tag, SNAP-tag and cell surface-specific ACP/MCP-tag systems can specifically label subpopulations of target protein expressed on the cell surface using non-cell permeable substrates (3).  This approach permits discrimination of different populations of a cell surface protein: those properly translocated to the plasma membrane from those retained in the secretory pathway or already internalized (e.g. upon ligand binding).

    References

    1. Lacapère J-J, Pebay-Peyroula E, Neumann J-M, Etchebest C. (2007) Trends Biochem Sci. 32, 259–270. PMID: 17481903
    2. von Heijne G. (2007) J Intern Med. 261, 543–557. PMID: 17547710
    3. Keppler, A., Pick, H., Arrivoli, C. et al. (2004) Proc. Natl. Acad. Sci. USA, 101, 9955. PMID: 15226507

    CLIP-tag™ is a trademark of New England Biolabs, Inc.
    SNAP-tag® is a registered trademark of New England Biolabs, Inc.

    • Fluorescent Labeling of COS-7 Expressing SNAP-tag® Fusion Proteins for Live Cell Imaging

      Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.

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    • SNAP-tag® Overview Tutorial

      View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.

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    Featured Products

    FAQs for CLIP Surface

    Features

    • Clone and express once, then use with a variety of substrates
    • Non-toxic to living cells
    • Wide selection of fluorescent substrates
    • Highly specific covalent labeling
    • Simultaneous dual labeling

    Applications

    • Simultaneous dual protein labeling on the surface of live cells
    • Protein localization and translocation
    • Pulse-chase experiments
    • Receptor internalization studies
    • Selective cell surface labeling
    • Protein detection in SDS-PAGE
    • Flow cytometry
    • High throughput binding assays in microtiter plates
    • Biosensor interaction experiments
    • FRET-based binding assays
    • Single molecule labeling
    • Super-resolution microscopy

    Protein Labeling with SNAP-tag and CLIP-tag

    The SNAP- (gold) or CLIP-tag (purple) is fused to the protein of interest (blue). Labeling occurs through covalent attachment to the tag, releasing either a guanine or a cytosine moiety.

    SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart

    NEB offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins.