The non-overlapping substrate specificity of SNAP-tag® and CLIP-tag™ enables the orthogonal and complementary labeling of two proteins simultaneously in the same cell. Furthermore, SNAP-tag and CLIP-tag based pulse-chase experiments allow for visualization of different generations of proteins and analysis of protein turnover in living cells (1-3). Additionally, there are a numbers of companion products designed to enable a wide variety of other applications including: Biotin substrates, SDS-PAGE specific SNAP-tag and CLIP-Vista labels, non-fluorescent blocking agents, resin and magnetic beads for pull-down applications, building blocks for the generation of custom substrates, and a SNAP-tag specific purified protein and antibody.
- Jansen, L. et al (2007) J.Cell. Biol. 176, 795-805. PMID: 17339380
- Farr, G.A. (2009) J. Cell. Biol. 186, 269-282. PMID: 19620635
- Milenkovic, L. (2010) J. Cell. Biol. 187, 365-374. PMID: 19948480
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, Inc.