Cellular Analysis

ACP/MCP Companion

Having no cysteines, the ACP-tag and the MCP-tag are particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies. Additionally, the CoA biotin substrate, a non-cell permeable substrate based on biotin with an amidocaproyl linker, is suitable for applications such as biotinylation of ACP-tag or MAC-tag fusion proteins in living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western Blot or for capture with streptavidin for binding and interaction studies.

SNAP-tag® is a registered trademark of New England Biolabs, Inc.

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FAQs for ACP/MCP Companion

Advantages

  • Simultaneous dual protein labeling on the surface of live cells
  • Protein localization and translocation
  • Pulse-chase experiments
  • Receptor internalization studies
  • Selective cell surface labeling
  • Protein detection in SDS-PAGE
  • Flow cytometry
  • High throughput binding assays in microtiter plates
  • Biosensor interaction experiments
  • FRET-based binding assays
  • Single molecule labeling
  • Super-resolution microscopy

Protein Labeling with ACP-tag

ACP-tag (red) fused to the protein of interest (blue) is labeled in the presence of a required synthase.

SNAP-tag®, CLIP-tag™ and ACP/MCP-tag Substrate Selection Chart

NEB offers a large selection of fluorescent labels (substrates) for SNAP-, CLIP-, ACP- and MCP-tag fusion proteins.