Gone are the days of buffer compatibility analyses and sub-optimal enzyme conditions! High Fidelity (HF™) Restriction Enzymes are all 100% active in a single buffer: CutSmart Buffer (NEB #B7004). This simplifies double digest reactions and eliminates the need for setting up sequential digests. HF enzymes share the same site specificity and cleavage efficiency as their wild-type counterparts, but offer the increased flexibility and convenience that you need in your laboratory. Whether you have 5 minutes or want to leave your reactions overnight, restriction enzymes digests with HF enzymes produce the same, reliable results. Set up your digests with reaction-specific enzymes concentration, without fear of risking product integrity to star activity. HF enzymes offer added flexibility and functionality without added cost -- they’re available for the same price-per-unit as the standard version.
HF™ is a trademark of New England Biolabs, Inc.
The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.