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We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,000 publications (as of 1/17) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
2005 Preparation of an immunoadsorbent coupled with a recombinant antigen to remove anti-acetylcholine receptor antibodies in abnormal serum. J.Immunol.Methods Guo, C.Y., Li, Z.Y., Xu, M.Q.and Yuan, J.M.
2005 A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level J. Am. Chem. Soc. Sando, S., Kanatani, K., Sato, N., Matsumoto, H. Hohsaka, T. and Aoyama, Y.
2005 Phylogenetic detection of conserved gene clusters in microbial genomes. BMC Bioinformatics Zheng, Y., Anton, B.P., Roberts, R.J.and Kasif, S.
2005 Productive interaction of chaperones with substrate protein domains allows correct folding of the downstream GFP domain. Gene Zhang, A., Cantor, E.J., Barshevsky, T.and Chong, S.
2005 Parasitic nematodes have two distinct chitin synthases. Mol.Biochem.Parasitol. Foster, J.M., Zhang, Y., Kumar, S.and Carlow, C.
2005 Protein Splicing Elements and Plants: From Transgene Containment to Protein Purification. Plant Biol. Evans, T.C., Jr., Xu, M.-Q.and Pradhan, S.
2005 Protein chemistry on the surface of living cells Chembiochem. Johnsson N. et al.
2005 Protein-functionalized polymer brushes Biomacromolecules Tugulu S. et al.
2005 Novel carboxylated N-glycans contain oligosaccharide-linked glutamic acid Biochem. Biophys. Res. Commun. Srikrishna, G., Brive, L., Freeze, H.H.
2005 Kluyveromyces lactis LAC4 Promoter Variants That Lack Function in Bacteria but Retain Full Function in K.lactis. Appl.Environ.Microbiol. Colussi, P.A.and Taron, C.H.
2005 Live chemical reports from the cell surface Chem. Biol. Cravatt B.F.
2005 Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis Appl. Environ. Microbiol. Colussi, P.A. and Taron, C.H.
2005 Measurement of the contributions of 1-D and 3-D pathways to the translocation of a protein along DNA. Proc.Natl.Acad.Sci.USA Gowers, D.M., Wilson, G.G.and Halford, S.
2005 N-Glycans of Caenorhabditis elegans are specific to developmental stages J. Biol. Chem. Cipollo, J.F., Awad, A.M., Costello, C.E., Hirschberg, C.B.
2005 Multicolor imaging of cell surface proteins J. Am. Chem. Soc. Vivero-Pol L. et al.
2005 Mining nematode genome data for novel drug targets. Trends Parasitol. Foster, J.M., Zhang, Y., Kumar, S.and Carlow, C.K.S.
2005 Protein synthesus by pure translation systems Methods Shimizu, Y. Kanamori, T. and Ueda, T.
2005 The chitin synthase genes chs-1 and chs-2 are essential for C.elegans development and responsible for chitin deposition in the eggshell and pharynx, respectively. Dev.Biol. Zhang, Y., Foster, J.M., Nelson, L.S., Ma, D.and Carlow C.K.S.
2005 Structure of HinP1I endonuclease reveals a striking similarity to the monomeric restriction enzyme MspI. Nucl.Acids.Res. Yang, Z., Horton, J.R., Maunus, R., Wilson, G.G., Roberts, R.R.and Cheng, X.
2005 SNAP-tag mediated live cell labeling as an alternative to GFP in anaerobic organisms BioTechniques Regoes A. et al.
2005 The mechanisms of intein-mediated protein splicing: variations on a theme. IUBMB Life Mills, K.V.and Perler, F.B.
2005 Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum Protein Expr. Purif. Goodin, J. L., et al.
2005 Two crystal forms of the restriction enzyme MspI-DNA complex show the same novel structure. Protein Sci. Xu, Q.S., Robert, R.J.and Guo, H.-C.
2005 The Wolbachia Genome of Brugia malayi: Endosymbiont Evolution within a Human Pathogenic Nematode. PLoS Biology Foster, J., Ganatra, M., Kamal, Ibrahim, Ware, J., Makarova, K., Ivanova, N., Bhattacharyya, A., Kapatral, V., Kumar, S., Posfai, J., Vincze, T., Ingram, J., Moran, L., Lapidus, A., Omelchenko, M., Kyrpides, N., Ghedin, E., Wang, S., Goltsman, E., Joukov, V., Ostrovskaya, O., Tsukerman, K., Mazur, M., Comb, D., Koonin, E.and Slatko, B.
2005 Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI. J.Mol.Biol. Heiter, D.F., Lunnen, K.D.and Wilson, G.G.
2005 Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly. FEBS Journal Fabre, A-L, Orlean, P.and Taron, C.H.
2005 Recent Advances in Protein Splicing: Manipulating Proteins In Vitro and In Vivo. Curr.Opin.Biotech. Xu, M.Q.and Evans, T.C.Jr.
2005 REBASE - restriction enzymes and DNA methyltransferases. Nucl.Acids.Res. Roberts, R.J., Vincze, T., Posfai, J.and Macelis, D.
2005 Self-cleavable stimulus responsive tags for protein purification without chromatography J. Am. Chem. Soc Ge, X., et. al.
2005 Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing Bioorg. Med. Chem. Lett. Girish, A., et al.
2005 Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling Chem. Biol Yin J. et al.
2005 Sequence Specificity and Role of Proximal Amino Acids of the Histone H3 Tail on Catalysis of Murine G9a Lysine 9 Histone H3 Methyltransferase. Biochemistry Chin, H.G., Pradhan, M., Esteve, P., Patnaik, D., Evans, T.C.Jr.and Pradhan, S.
2005 Characterization of a Nucleus-Encoded Chitinase from the Yeast Kluyveromyces lactis. Appl.Environ.Microbiol. Colussi, P.A., Specht, C.A.and Taron, C.H.
2005 Characterization of the Type III restriction endonuclease PstII from Providencia stuartii. Nucl.Acids Res. Sears, A., Peakman, L.J., Wilson, G.G.and Szczelkun, M.D.
2005 Characterization of a nucleus-encoded chitinase from the yeast Kluyveromyces lactis Appl. Environ. Microbiol. Colussi, P.A., Specht, C.A. and Taron, C.H.
2005 Characterization of Two New Aminopeptidases in Escherichia coli. BMC Bioinformatics Zheng, Y., Roberts, R.J., Kasif, S.and Guan, C.
2005 Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides J. Biol. Chem. Ying, B.W., Taguchi, H., Kondo, M. and Ueda, T.
2005 Cleavage of individual DNA strands by the different subunits of the heterodimeric restriction endonuclease BbvCI. J.Mol.Biol. Bellamy, S.R.W., Milsom, S.E., Scott, D.J., Daniels, L.E., Wilson, G.G.and Halford, S.E.
2005 Chracterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J.Biol.Chem. An.L., Tang, W., Ranalli, T.A., Kim, H-J., Wytiaz, J.and Kong, H.
2005 Automated isolation techniques for microalgae, pgs.101-116 in "Algal culturing techniques" Algal culturing techniques Sieracki, M., Poulton, N., Crosbie, N.
2005 A versatile system for the expression of nonmodified bacteriocins inEscherichia coli J. Appl. Microbiol. Ingham, A.B., et al.
2005 A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA. EMBO J. Vanamee, E.S., Viadiu, H., Kucera, R., Dorner, L., Picone, S., Schildkraut, I.and Aggarwal, A.K.
2005 Analysis and function of prototype foamy virus envelope N glycosylation J. Virol.  Lüftenegger, D., Picard-Maureau, M., Stanke, N., Rethwilm, A., Lindemann, D.
2005 Alternative luciferase for monitoring bacterial cells under adverse conditions. Appl. Environ. Microbiol. Wiles, S., Ferguson, K., Stefanidou, M., Young, D.B. and Robertson, B.D.
2005 Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo Mol Ther. Tannous, B.A., Kim, D.E., Fernandez, J.L., Weissleder, R. and Breakefield, X.O.
2005 Harnessing Inteins for Protein Purification and Characterization. Nucleic Acids and Molecular Biology Chong, S.and Xu.M.Q.
2005 Alternative luciferase for monitoring bacterial cells under adverse conditions  Appl. Environ. Microbiol. Wiles, S., Ferguson, K., Stefanidou, M., Young, D.B. and Robertson, B.D.
2005 Functional analysis of the N- and C-terminus of mammalian G9a histone H3 methyltransferase. Nucl.Acids Res. Esteve, P.O., Patnaik, D., Chin, H.G., Benner, J., Teitell, M.A.and Pradhan, S.
2005 How restriction enzymes became the workhorses of molecular biology. PNAS Roberts, R.J.
2005 Expression of SMAP-29 cathelicidin-like peptide in bacterial cells by intein-mediated system Protein Expr. Purif. Morassutti, C., et al.
2005 Labeling proteins with small molecules by site-specific posttranslational modification J Am Chem Soc Yin J. et al.
2005 Human maintenance DNA (cytosine-5)-methyltransferase and p53 modulate expression of p53-repressed promoters. Proc.Natl.Acad.Sci.USA Esteve, P.O., Chin, H.G.and Pradhan, S.
2005 Implications for Switching Restriction Enzyme Specificities from the Structure of BstYI Bound to a BglII DNA Sequence Structure Townson, S.A., Samuelson, J.C., Xu, S.Y.and Aggarwal, A.K.
2005 Explosive Lineage-Specific Expansion of the Orphan Nuclear Receptor HNF4 in Nematodes. J Mol Evol. Robinson-Rechavi, M, Maina, C.V., Gissendanner, C.R., Laudet, V., Sluder, A.
2005 Crystal structure of the restriction-modification system control element C.BclI and mapping of its binding site. Structure Sawaya, M.R., Zhu, Z., Fana, M., Chan, S.H., Dabur, R., Xu, S.Y.and Balendiran, G.K.
2005 Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy Eur. Biophys. J Kufer S.K. et al.
2005 Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo Mol Ther. Tannous, B.A., Kim, D.E., Fernandez, J.L., Weissleder, R., Breakefield, X.O.
2005 Crystal structures of an intein from the split dnaE gene of Synechocystis sp.PCC6803 reveal the catalytic model of intein without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing. J.Mol.Biol. Ping, S., Ye, S., Ferrandon, S., Evans, T.C., Xu, M-Q.and Rao, Z.
2005 Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells ChemBioChem Juillerat A. et al.
2005 DNA -binding activity of LndI protein and temporal expression of the gene that upregulates landomycin E production in Streptomyces globisporus 1912 Microbiology Rebets, Y., et al.
2005 Sau421, a Bcgl-like restriction-modification system encoded by the Staphylococcus aureus quadruple-converting phage phi 42 Microbiology Dempsey, R.M., Carroll, D., Kong, H.M., Higgins, L., Keane, C.T., Coleman, D.C.
2005 Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrance proteins Biotechnol, Prog. Kuruma, Y., Nishiyama, K., Shimizu, Y., Muller, M and Ueda, T.
2005 Specificity changes in the evolution of Type II restriction endonucleases- A Biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences J Biol. Chem. Pingoud, V., Sudina, A., Geyer, H., Bujnicki, J.M., Lurz, R., Luder, G., Morgan, R., Kubareva, E., Pingoud, A.
2006 A highly sensitive protein-protein interaction assay based onGaussia luciferase Nat. Methods Remy, I. and Michnick, S.W.
2006 A highly sensitive protein-protein interaction assay based onGaussia luciferase Nat. Methods Remy, I. and Michnick, S.W.
2006 A highly sensitive protein-protein interaction assay based on Gaussia luciferase. Nat. Methods Remy. I. and Michnick, S.W.
2006 A genetic dissection of the LlaJI restriction cassette reveals insights on a novel bacteriophage resistance system. BMC Microbiol. O'Driscoll, J., Heiter, D.F., Wilson, G.G., Fitzgerald, G.F., Roberts, R.and van Sinderen, D.
2006 Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism. J Biol Chem. Joshi, H.K., Etzkorn, C., Chatwell, L., Bitinaite, J.and Horton, N.C.
2006 Adding value to fusion proteins through covalent labeling Curr. Opin. Biotechn. Gronemeyer T. et al.
2006 Protein function microarrays based on self-immobilizing and self-labeling fusion proteins ChemBioChem. Sielaff I. et al.
2006 Protein function microarrays based on self-immobilizing and self-labeling fusion proteins ChemBioChem. Sielaff I. et al.
2006 Protein splicing in cis and in trans. The Chemical Record Saleh, L.and Perler, F.B.
2006 Properties of the C-terminal domain of enzyme I of the Escherichia coli phosphotransferase system. J Biol Chem. Patel, H.V., Vyas, K.A., Mattoo, R.L., Southworth M., Perler, F.B., Comb, D.and Roseman, S.
2006 Reactivity of intein thioesters: appending a functional group to a protein Chembiochem. Kalia, J., et al.
2006 Protein splicing mechanisms and applications. IUBMB Life Perler, F.B.
2006 Real-time observation of trigger factor function on translating ribosomes Nature Kaiser, C.M., Chang, H.C., Agashe, V.R., Lakshmipathy, S.K., Etchells, S.A., Hayer-Hartl, M., Hartl, F.U. and Barral, J.M.
2006 It Takes Two: Lessons From the First Nematode Wolbachia Genome Sequence. Issues in Infectious Disease Pfarr, K., J.Foster and B Slatko
2006 Intein-mediated in vitro synthesis of lipidated Ras proteins Chem. Commun. (Camb) Gottlieb, D., et al.
2006 In vivo characterization of the GPI assembly defect in yeast mcd4-174 mutants and bypass of the Mcd4p-dependent step in delta mcd4 cells. FEMS Yeast Res. Wiedman, J.M., Fabre, A-L., Taron, B.W., Taron, C.H.and Orlean, P.
2006 LT CI, a novel chymotrypsin inhibitor of the potato I family from the earthworm Lumbricus terrestris. Purification, cDNA cloning, and expression Comp. Biochem. Physiol B. Biochem. Mol. Biol. Wojtaszek, J., et al.
2006 Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro Nat. Protoc. Demidov, V.V., and Broude, N.E.
2006 Production and purification of recombinant human glucagons overexpressed as intein fusion protein in Escherichia coli Protein Pept. Lett. Esipov, R.S., et. al.
2006 Post-translational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells ChemBioChem Prummer M. et al.
2006 RecJ exonuclease: substrates, products and interaction with SSB. NAR Han, E.S., Cooper, D.L., Persky, N.S., Sutera, V.A.Jr., Whitaker, R.D., Montello, M.L.and Lovett, S.T.
2006 The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein.GP-C. Virol. J. Eichler, R., Lenz, O., Garten, W., Strecker, T.
2006 The N terminus of GTP gamma S-activated transducin alpha-subunit interacts with the C terminus of the cGMP phosphodiesterase gammasubunit J. Biol. Chem.  Grant, J., et al.
2006 Synthesis and evaluation of the bioluminescent activity of (S)-Cypridina luciferin and its analogs Tetrahedron Letters Wu, C., Kawasaki, K., Ohgiya, S., Ohmiya, Y.
2006 The role of Wolbachia in the biology and pathogenesis of filariasis Insect Symbiosis Turner, J., Foster, J., Ganatra, M., Slatko, B.and Taylor, M.
2006 TspMI, a thermostable isoschizomer of XmaI (5'C/CCGGG3'): characterization and single molecule imaging with DNA. Appl Microbiol.Biotechnol. Parashar, V., Capalash, N., Xu, S.Y., Sako, Y.and Sharma, P.
2006 Visualizing receptor trafficking in living PNAS Jacquier V. et al.
2006 Semisynthesis and characterization of mammalian thioredoxin reductase Biochem. Eckenroth, B., et al.
2006 Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome. Nucl.Acids.Res. Xu, Y., Kim, H-J., Kays, A., Rice, J.and Kong, H.
2006 Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address  Proteomics Jongsma M.A., Litjens R. H.
2006 Syntheses and evaluation of the bioluminescent activity of (S)-Cypridina luciferin and its analogs Tetrahedron Letters Wu, C., Kawasaki, K., Ohgiya.S. and Ohmiya, Y.
2006 Structure and dynamics of the epidermal growth factor receptor C-terminal phosphorylation domain Protein Sci. Lee, N.Y., et al.
2006 SmpB triggers GTP hydrolysis of elongation factor Tu on ribosome by compensating for the lack of codon-anticodon interaction during trans-translation initiation J. Biol. Chem. Shimizu, Y. and Ueda, T.
2006 In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis Biochemistry O’Reilly, M.K., Zhang, G., Imperiali, B.
2006 Cofactor-independent Phosphoglycerate Mutase is an essential gene in procyclic form Trypanosoma brucei. Parasitol.Res. Djikeng, A., Raverdy, S., Foster, J., Bartholomeu, D., Zhang, Y., El-Sayed, N.and Carlow, C.
2006 Cloning of Nt.CviQII nicking endonuclease and its cognate methyltransferase: M.CviQII methylates AG sequences. Protein Expr.Purif. Chan, S.H., Zhu, Z., Van Etten, J.L.and Xu, S.Y.
2006 Catalytic properties and kinetic mechanism of human recombinant Lys-9 histone H3 methyltransferase SUV39H1: participation of the chromodomain in enzymatic catalysis. Biochemistry Chin, H.G., Patnaik, D., Esteve, P.O., Jacobsen, S.E.and Pradhan.S.