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We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,000 publications (as of 1/17) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
2001 AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucl.Acids Res. Cheng, X.and Roberts, R.J.
2001 Characterization of a naturally occurring trans-splicing intein from Synechocystis sp.PCC6803. Biochemistry Martin, D.D., Xu, M-Q and Evans, T.C.Jr.
2001 Cell-free translation reconstituted with purified components Nat. Biotech. Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K. and Ueda, T.
2001 Protein trans-Splicing To Produce Herbicide-Resistant Acetolactate Synthase. Appl.Environ.Microbiol. Sun, L., Ghosh, I., Paulus, H.and Xu, M.Q.
2001 Protein splicing and its aplications. Genet.Eng.(NY) Giriat, I., Muir, T.W.and Perler, F.B.
2001 Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. BioTechniques Southworth, M.W., Amaya, K., Evans, T.C., Xu, M-Q and Perler, F.B.
2001 PubMed Central: The GenBank of the Published Literature. Proc.Natl.Acad.Sci. Roberts, R.J.
2001 Protein splicing. Encyclopedia of Genetics Xu, M.Q., Chong, S.and T.C.Evans, Jr.
2001 Intein-mediated rapid purification of cre recombinase Protein Expr.Purif. Cantor, E.J.and Chong, S.
2001 Intein-Mediated Ligation and Cyclization of Expressed Proteins. Methods Xu, M.Q.and T.C.Evans, Jr.
2001 Nuclear receptors in nematodes: themes and variations. Trends in Genetics Sluder, A.E.and Maina, C.V.
2001 Molecular Biology Problem Solver: A Laboratory Guide Bonventre, J., Walsh, P.and Robinson, D.
2001 The sequence of the human genome Science Venter, J.C., Roberts, R.J.et al.
2001 The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI. Nucl.Acids Res. Higgins, L.S., Besnier, C.and Kong H.
2001 Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein. J.Biol.Chem. Ghosh, I., Sun, L.and Xu, M.Q.
2001 The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability J. Gen. Virol. Dorsch, S., et al.
2001 The Energetics of the Interaction of BamHI Endonuclease with its Recognition Site GGATCC. J.Mol.Biol. Engler, L.E., Sapienza, P., Dorner, L.F., Kucera, R., Schildkraut, I.and Jen-Jacobson, L.
2001 REBASE-restriction enzymes and methylases. Nucl.Acids Res. Roberts, R.J., and Macelis, D.
2001 Qualitative detection of apoptotic cells assessed by DNA fragmentation. Methods Mol.Biol. McIlwrath, A.J.
2001 Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis. Structure van der Woerd, M.J., Pelletier, J.J., Xu, S.and Friedman, A.M.
2001 Recombinant human DNA (cytosine-5) methyltransferase.III.Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGGCCG sequence. J.Biol.Chem. Bacolla, A., Pradhan, S., Larson, J.E., Roberts, R.J.and Wells, R.D.
2001 Construction of high-complexity combinatorial phage display peptide libraries. Methods Noren, K.A.and Noren, C.J.
2001 Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene Zhang, A., Gonzales, S.M., Cantor, E.J.and Chong, S.
2001 Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling Mol Cell. Wu, J.W., et al.
2001 Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state. EMBO Reports Besnier, C.E.and Kong, H.
2001 Comparative genomics of the restriction-modification systems in Helicobacter pylori. Proc.Natl.Acad.Sci.USA Lin, L.F., Posfai, J., Roberts, R.J.and Kong, H.
2001 Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng. Kalidas, C., Joshi, L., Batt, C.
2001 Hyperthermophilic Inteins. Methods Enzymol. Perler, F.B.
2001 Hybridization of High-Denisty Filter Arrays of a Brugia malayi BAC Library with Biotinylated Oligonucleotides and PCR Products. BioTechniques Foster, J.M., Kamal, I.H., Daub, J., Swan, M.C., Ingram, J.R., Ganatra, M., Ware, J., Guiliano, D., Aboobaker, A., Moran, L., Blaxter, M.and Slatko, B.E.
2001 Information access.Building a "GenBank" of the published literature. Science Roberts, R.J., Varmus, H.D., Ashburner, M., Brown, P.O., Eisen, M.B., Khosla, C., Kirschner, M., Nusse, R., Scott, M.and Wold, B.
2001 In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore Proc. Natl. Acad. Sci. USA Hanzawa, H., et al.
2001 Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene Chen, L., Pradhan, S.and Evans, T.C.Jr.
2001 Determination of Wolbachia Genome Size by Pulsed-Field Gel Electrophoresis. J.Bacteriol. Sun, L.V., Foster, J.M., Tzertzinis, G., Ono, M., Bandi, C., Slatko, B.E.and O'Neill, S.L.
2001 Detection of Serum IgG Antibodies Specific for Wolbachia Surface Protein in Rhesus Monkeys Infected with Brugia malayi. J Infectious Diseases Punkosdy, G.A., Dennis, V.A., Lasater, B.L., Tzertzinis, G.foster, J.M.and Lammie, P.J.
2001 Engineering a Nicking Endonuclease N.AlwI by Domain Swapping Proc.Natl.Acad.Sci.USA Xu, Y., Lunnen, K.and Kong, H.
2001 Discovery and distribution of super-integrons among pseudomonads. Mol.Microbiol. Vaisvila, R., Morgan, R.D., Posfai, J.and Raleigh, E.A.
2001 Restriction endonucleases. In: Molecular biology problem solver Wiley-Liss, Inc. Robinson, D., Walsh, P.R., Bonventre, J.A.
2001 The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA J. Mol. Biol. Fatemi, M., Hermann, A., Pradhan, S., Jeltsch, A.
2001 Structural study of restriction enzyme MspI/DNA complex by x-ray crystallography Biophys. J. Xu, Q., O'Loughlin, T.J., Kucera, R.B., Schildkraut, I., Guo, H.-C.
2002 Acyclic and dideoxy teminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases. Nuc.Acids Res. Gardner, A.F.and Jack, W.E.
2002 A novel cyclophilin from parasitic and free-living nematodes with a unique substrate-and drug-binding domain. J.Biol.Chem. Ma, D., Nelson, L.S., LeCoz, K., Poole, C.and Carlow, C.K.S.
2002 Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity Protein Sci Wojciechowski CL, Cardia JP, and Kantrowitz ER.
2002 Merozoite surface protein-9 of Plasmodium vivax and related simian malaria parasites is orthologous to p101/ABRA of P.falciparum. Mol.Biochem.Parasitol. Vargas-Serrato, E., Barnwell, J.W., Ingravallo, P., Perler, F.B.and Galinski, M.R.
2002 Mechanistic and kinetic considerations of protein splicing. Chem.Rev. Evans, T.C., Jr.and Xu, M-Q
2002 Modulation of postendocytic sorting of G protein-coupled receptors Science Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M.
2002 Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein. J.Bacteriol. Southworth, M.W.and Perler, F.B.
2002 Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis J. Bacteriol Nakano, S., et al.
2002 InBase, the Intein Database. Nuc.Acids Res. Perler, F.B.
2002 Helminth genomics: from gene discovery to genome sequencing. Trends Parasitol Foster, J.M.and Johnston, D.A.
2002 Inhibition of poly(ADP-ribosyl)ation induces DNA hypermethylation: a possible molecular mechanism. FASEB J. Zardo, G., Reale, A., Passananti, C., Pradhan, S., Bountempo, S., DeMatteis, G., Adams, R.L.P.and Caiafa, P.