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We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,000 publications (as of 1/17) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
Screening of genetic parameters for soluble protein expression in Escherichia coli Protein Expression and Purification Vernet, E., Kotzsch, A., Voldborg, B., et al.
Selection of a mimotope peptide of S-adenosyl-l-homocysteine and its application in immunoassays Molecules Wu, C. and Tzertzinis, G. Phage Display
The level of synthesis and secretion of Gaussia princeps
Identification of a cardiac specific protein transduction domain by in vivo biopanning using a M13 phage peptide display library in mice PLoS One Zahid, M. et al.
Targeting of tumor blood vessels
Production of disulfide-bonded proteins in Escherichia coli Berkmen, M.
In vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi Parasites and Vectors Landmann, F., Foster, J.M., Slatko, B.E. and Sullivan, W.
"Bacterial Systems" introduction of Membrane Proteins - Strategies for Expression and Isolation Samuelson, J.C.
Near-Infrared Fluorescence Imaging of Mammalian Cells and Xenograft Tumors with SNAP-tag PLoS ONE Gong, H.; Kovar, J. L.; Baker, B.; Zhang, A.; Cheung, L.; Draney, D. R.; Corrêa, I. R., Jr.; Xu, M.-Q.; Olive, M. D.
Database of DNA polymerases Curr Protoc Mol Biol. Langhorst, B.W. and Nichols, N.M.
waffles
Optimization of Medium Composition for Production pf Recombinant
An insight into the various regulatory mechanisms modulating human DNA methyltransferase 1 stability and function Epigenetics Kar, S., Deb, M., Sengupta, D. Shilpi, A., Parbin, S., Torrisani, J., Pradhan, S. and Patra, S.
Rapid Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components
Structural basis for human PRDM9 action at recombination hot spots. Genes Dev. Patel, A., Horton, J.R., Wilson, G.G., Zhang, X. and Cheng, X.
Culture-independent genomic characterisation of Candidatus Chlamydia
Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella
1980 The Evolution of Genes: the Chicken Preproinsulin Gene Cell Perler, F.B.and Efstratiadis, A.
1980 Restriction endonucleases from three strains of Haemophilus influenzae Sci. Sin. Shen, S.X., Li, Q.L., Yan, P,F., Zhou, B., Ye, S.Y., Lu, Y.Y., Wang, D.B.
1981 Two new restriction endonucleases from Proteus vulgaris. Nucl.Acids Res. Gingeras, T.R., Greenough, L., Schildkraut, I., and Roberts, R.J.
1982 NdeI: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5'-CATATG-3' sequences. FEBS Lett. Watson, R.J., Schildkraut, I., Qiang, B.Q., Martin, S.M., and Visentin, L.P.
1983 Analysis of Regulation of phoB Expression Using a phoB-cat Fusion. J.Bacteriol. Guan, C., Wanner, B., and Inouye, H.
1983 The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene Nucleic Acids Res. Brooks, J.E., Blumenthal, R.M., Gingeras, T.R.
1984 Alteration of apparent restriction endonuclease recognition specificities by DNA methylases. Nucl.Acids Res. Nelson, M., Christ, C., and Schildkraut, I.
1984 Screening for and characterizing restriction endonucleases Genetic Engineering Schildkraut, I.
1984 DNA modification methylases: New uses in the manipulation of DNA. BioTechniques Christ, C., and Nelson, M.
1984 The cleavage site for the restriction endonuclease EcoRV is 5'-GAT^ATC-3'. Gene Schildkraut, I., Banner, C.D.B., Rhodes, C.S., and Parekh, S.
1984 Acetylcholinesterase secreted by intestinal nematodes: a reinterpretation of its putative role of "biochemical holdfast." Trans.Royal Soc Trop.Med.and Hyg. Philipp, M.
1984 A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus. Nucl.Acids.Res. Qiang, B.Q., and Schildkraut, I.
1985 Nuclear and Mitochondrial DNA of the Primate Malarial Parasite Plasmodium Knowlesi. Mol.Biochem.Parasitol. Williamson, D.H., Wilson, R.J., Bates, P.A., McCready, S., Perler, F., and Qiang, B.Q.
1985 Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products. Nucl.Acids Res. Theriault, G., Roy, P.H., Howard, K.A., Benner, J.S., Brooks, J.S., Waters, A.F., and Gingeras, T.R.
1985 The effect of site specific methylation on restriction endonuclease digestion. Nucl.Acids Res. McClelland, M., and Nelson, M.
1985 Purification of MboII methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences. Nucl.Acids Res. McClelland, M., Nelson, M., and Cantor, C.R.
1986 Sequencing in the Fast Lane: A Rapid Protocol for [a-35S] dATP Dideoxy DNA Sequencing. BioTechniques Williams, S.A., Slatko, B.E., Moran, L.S., and DeSimone, S.M.
1986 Two unique restriction endonucleases from Neisseria lactamica. Nucl.Acids Res. Qiang, B.Q., and Schildkraut, I.
1986 Escherichia coli K-12 restricts DNA containing 5-methylcytosine. Proc.Natl.Acad.Sci.USA Raleigh, E.A., and Wilson, G.
1986 Biochemical and immunologic characterization of a major surface antigen of Dirofilaria immitis infective larvae. J.Immunol. Philipp, M., and Davis, T.B.
1986 Cloning and comparison of repeated DNA sequences from the human filarial parasite Brugia malayi and the animal parasite Brugia pahangi. Proc.Natl.Acad.Sci.USA McReynolds, L.A., DeSimone, S.M., and Williams, S.A.
1986 Cloning and characterization of two Onchocerca volvulus repeated DNA sequences. Mol.Biochem.Parasitol. Perler, F.B., and Karam, M.
1986 Cloning the DdeI restriction-modification system using a two-step method. Nucl.Acids Res. Howard, K.A., Card, C., Benner, J.S., Callahan, H.L., Maunus, R., Silber, K., Wilson, G., and Brooks, J.E.
1987 NotI and SfiI: restriction endonucleases with octanucleotide recognition sequences. Methods Enzymol. Qiang, B.-Q., and Schildkraut, I.
1987 N4-Methylcytosine as a minor base in bacterial DNA. J.Bacteriol. Ehrlich, M., Wilson, G.G., Kenneth, C.K., and Gehrke, C.W.
1987 Properties and uses of restriction endonucleases. Methods Enzymol. Brooks, J.E.
1987 Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein. Nucl.Acids Res. Sznyter, L.A., Slatko, B., Moran, L., O'Donnell, K.H., and Brooks, J.E.
1987 Monoclonal antibody to a unique surface epitope of the human filaria Brugia malayi identifies infective larvae in mosquito vectors. Proc.Natl.Acad.Sci.USA Carlow, C.K.S., Franke, E.D., Lowrie, R.C.J., Partono, F., and Philipp, M.
1987 Highly Repeated DNA Sequences as Species-specific Probes for Brugia. Parasitology Today Piessens, W.F., McReynolds, L.A.and Williams, S.A.
1987 Genomic Cloning of Recombinant Antigens Detected by Antiserum Against Infective Larvae of Brugia Malayi. Molecular Paradigms for Eradicating Helminthic Parasites Arasu, P., and Perler, F.B.
1987 Monoclonal antibodies to parasite antigens: a rapid immunization protocol requiring small numbers of parasites. J.Parasitol. Carlow, C.K.S., Edwards, M.K., James, E.R., and Philipp, M.
1987 Isolation and Identification of restriction endonuclease BstFI Nucl.Acids Res. Kong, H and Chen, Z.
1987 The use of DNA methylases to alter the apparent recognition specificities of restriction endonucleases. Methods Enzymol. Nelson, M., and Schildkraut, I.
1987 The effect of site-specific methylation on restriction-modification enzymes. Nucl.Acids Res. Nelson, M., and McClelland, M.
1987 The Use of DNA Sequencing Reactions as Probes for Southern Blots, Dot Blots and Colony Hydridizations. BioTechniques Card, C.O., Williams, S.A., Moran, L.S., Poole, C.B., and Slatko, B.E.
1987 The cleavage site for the restriction endonucleases BanI and HgiCI is 5' ...G^GPyPuCC ...3'. Nucl.Acids Res. Schildkraut, I., Lynch, J., and Morgan, R.
1987 Purification and assay of Type II DNA methylases. Methods Enzymol. Nelson, M., and McClelland, M.
1987 Protective immunity to Brugia malayi larvae in BALB/c mice: potential of this model for the identification of protective antigens. Am.J.Trop.Med.Hyg. Carlow, C.K.S., and Philipp, M.
1987 Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucl.Acids Res. McClelland, M., Jones, R., Patel, Y., and Nelson, M.
1987 Restriction and modification in vivo by Escherichia coli K12 Methods Enzymol. Raleigh, E.A.
1987 Cloning of two type II methylase genes that recognise asymmetric nucleotide sequences: FokI and HgaI. Mol.Gen.Genet. Nwankwo, D., and Wilson, G.
1987 Enhancement of the apparent cleavage specificities of restriction endonucleases: applications to megabase mapping of chromosomes. McClelland, M., and Nelson, M.
1987 Cloning and characterization of an abundant Plasmodium knowlesi antigen which cross reacts with Gambian sera. Mol.Biochem.Parasitol. Perler, F.B., Moon, A.M., Qiang, B.-Q., Meda, M., Dalton, M., Card, C., Schmidt-Ullrich, R., Wallach, D., Lynch, J., and Donelson, J.E.
1987 Cloning, sequencing and expression of the TaqI restriction-modification system. Nucl.Acids Res. Slatko, B.E., Benner, J.S., Jager-Quinton, T., Moran, L.S., Simcox, T.G., VanCott, E.M., and Wilson, G.G.
1987 Cloning, sequencing, in vivo promoter mapping, and expression in Escherichia coli of the gene for the HhaI methyltransferase. J.Biol.Chem. Caserta, M., Zacharias, W., Nwankwo, D., Wilson, G.G., and Wells, R.D.
1987 Brugia malayi: recombinant antigens expressed by genomic DNA clones. Exp.Parasitol. Arasu , P., Philipp, M., and Perler, F.
1987 A unique type II restriction endonuclease from Acinetobacter lwoffi N. Nucl.Acids Res. Morgan, R.D., Dalton, M., and Stote, R.
1987 A Developmentally regulated surface epitope expressed by the infective larva of Brugia malayi which is rapidly lost after infection. Molecular Paradigms for Eradicating Helmenthic Parasites Carlow, C.K.S., Perrone, J., Spielman, A., and Philipp, M.A.
1987 Dirofilaria immitis: Genomic Complexity and Characterication of a Structural Gene. Molecular Paradigms for Eradicating Helminthic Parasites Maina, C.V., Grandea, A.G.I., Le, T.K.T., Nur, A., Williams, S.A., and McReynolds, L.A.
1988 AseI, a restriction endonuclease from Aquaspirillum serpens which recognizes 5'ATTAAT3'. Nucl.Acids Res. Polisson, C., and Morgan, R.D.
1988 An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene Maina, C.V., Riggs, P.D., Grandea, A.G.I., Slatko, B.E., Moran, L.S., Tagliamonte, J.A., McReynolds, L.A., and Guan, C.D.
1988 BsrI, a unique restriction endonuclease from Bacillus stearothermophilus which recognizes 5'ACTGG3'. Nucl.Acids Res. Polisson, C., and Morgan, R.D.
1988 Cloned restriction-modification systems - a review. Gene Wilson, G.G.
1988 A site-specific single strand endonuclease activity induced by NYs-1 virus infection of a Chlorella-like green alga. Nucl.Acids Res. Xia, Y., Morgan, R., Schildkraut, I., and Van Etten, J.L.
1988 Characterization of clones of the BamHI methyltransferase gene. Gene Nathan, P.D.and Brooks, J.E.
1988 Cloning and analysis of the HaeIII and HaeII methyltransferase genes. Gene Slatko, B.E., Croft, R., Moran, L.S., and Wilson, G.G.
1988 Cloning Type II restriction and modification genes. Gene Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., and Wilson, G.G.
1988 Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems. Gene Van Cott, E.M., and Wilson, G.G.
1988 EarI, a restriction endonuclease from Enterobacter aerogenes which recognizes 5'CTCTTC3'. Nucl.Acids Res. Polisson, C., and Morgan, R.D.
1988 IMMUNITY IN FILARIASIS: Perspectives for Vaccine Development. Ann.Rev.Microbiol. Philipp, M., Davis , T.B., Storey, N., and Carlow, C.K.S.
1988 Genetic dissection of the methylcytosine-specific restriction system mcrB of Escherichia coli K-12. Gene Dila, D., and Raleigh, E.A.
1988 Cloning and sequencing the HinfI restriction and modification genes. Gene Chandrasegaran, S., Lunnen, K.D., Smith, H.O., and Wilson, G.G.
1988 Cloning of the HhaI and HinPI restriction-modification systems. Gene Barsomian, J.M., Card, C.O., and Wilson, G.G.
1988 Cloning and expression of the MspI restriction and modification genes. Gene Nwankwo, D.O., and Wilson, G.G.
1988 Cloning, purification and characterization of the M.NdeI methyltransferase from Neisseria denitrificans Gene Silber, K.R., Polisson, C., Rees, P.A., and Benner, J.S.
1988 Cloning, purification and characterization of the HincII and HindII methyltransferases from Haemophilus influenzae. Gene Rees, P.A., Nwankwo, D.O., Wilson, G.G., and Benner, J.S.
1988 The characterization and cloning of the EagI restriction-modification system. Gene Sznyter, L.A., and Brooks, J.E.
1988 The Amino Acid Sequence of the Eukaryotic DNA [N6-adenine] Methyltransferase, M-CviBIII, Has Regions of Similarity With the Prokaryotic Isoschizomer M-TaqI and Other DNA [N6-adenine] Methyltransferases. Gene Narva, K.E., Van Etten, J.L., Slatko, B.E.and Benner, J.S.
1988 Type II restriction-modification systems. Trends in Genetics Wilson, G.G.
1988 Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene Guan, C.D., Li, P., Riggs, P.D., and Inouye, H.
1988 McrA and McrB restriction phenotypes of some E.coli strains and implications for gene cloning. Nucl.Acids Res. Raleigh, E.A., Murray, N.E., Revel, H., Blumenthal, R.M., Westaway, D., Reith, A.D., Rigby, P.W.J., Elhai, J., and Hanahan, D.
1988 Isolation and characterization of restriction endonuclease BstYI from Bacillus stearothermophilus. FEBS Lett. Chen Z.and Kong, H.
1988 MseI, a unique restriction endonuclease from Micrococcus species which recognizes 5'T^TAA 3'. Nucl.Acids Res. Morgan, R.D.
1988 Nomenclature for bacterial genes coding for class-II restriction endonucleases and modification methyltransferases. Gene Szybalski, W., Blumenthal, R.M., Brooks, J.E., Hattman, S., and Raleigh, E.A.
1988 Species-specific oligonucleotide probes for the identification of human filarial parasites. Mol.Biochem.Parasitol. Williams, S.A., DeSimone, S.M., and McReynolds, L.A.
1988 Cloning and characterization of the BamHI restriction modification system Gene Brooks, J.E., Benner, J.S., Silber, K.R., Heiter, D.F., Sznyter, L.A., Jager-Quinton, T., Wilson, G.G., Moran, L.S., Slatko, B.E., Nwankwo, D.O.
1988 Isolation and properties of a new site specific endonuclease Bme142I from Bacillus megaterium Nucleic Acids Res. Fomenkov, A.I., Kramarov, V.M., Andreev, L.V., Mochalov, V.V., Smolyaninov, V.V., Matvienko, N.I.
1988 The characterization and cloning of the NotI restriction-modification system Heredity Sznyter, L.A., Brooks. J.E.
1989 A l gt11 cDNA recombinant that encodes Dirofilaria immitis paramyosin. Mol.Biochem.Parasitol. Grandea, A.G.I., Le, T.K.T., Asikin, N., Davis, T.B., Philipp, M., Cohen, C., and McReynolds, L.A.
1989 Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei. Gene Lunnen, K.D., Morgan, R.D., Timan, C.J., Krzycki, J.A., Reeve, J.N., and Wilson, G.G.
1989 M.FokI methylates adenine in both strands of its asymmetric recognition sequence. Gene Landry, D., Looney, M.C., Feehery, G.R., Slatko, B.E., Jack, W.E., Schildkraut, I., and Wilson, G.G.
1989 Isolation and characterization of restriction endonuclease BstFI and BstSI from Bacillus stearothermophilus. J.Fudan Univ.Natural Sci. Chen Z., Kong, H.and Wang, L.
1989 MscI, a type II restriction endonuclease from Micrococcus species which recognizes 5' TGGCCA 3'. Nucl.Acids Res. Polisson, C.