We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,000 publications (as of 1/17) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
2001 AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucl.Acids Res. Cheng, X.and Roberts, R.J.
2001 Characterization of a naturally occurring trans-splicing intein from Synechocystis sp.PCC6803. Biochemistry Martin, D.D., Xu, M-Q and Evans, T.C.Jr.
2001 Cell-free translation reconstituted with purified components Nat. Biotech. Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K. and Ueda, T.
2001 Protein trans-Splicing To Produce Herbicide-Resistant Acetolactate Synthase. Appl.Environ.Microbiol. Sun, L., Ghosh, I., Paulus, H.and Xu, M.Q.
2001 Protein splicing and its aplications. Genet.Eng.(NY) Giriat, I., Muir, T.W.and Perler, F.B.
2001 Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. BioTechniques Southworth, M.W., Amaya, K., Evans, T.C., Xu, M-Q and Perler, F.B.
2001 PubMed Central: The GenBank of the Published Literature. Proc.Natl.Acad.Sci. Roberts, R.J.
2001 Protein splicing. Encyclopedia of Genetics Xu, M.Q., Chong, S.and T.C.Evans, Jr.
2001 Intein-mediated rapid purification of cre recombinase Protein Expr.Purif. Cantor, E.J.and Chong, S.
2001 Intein-Mediated Ligation and Cyclization of Expressed Proteins. Methods Xu, M.Q.and T.C.Evans, Jr.
2001 Nuclear receptors in nematodes: themes and variations. Trends in Genetics Sluder, A.E.and Maina, C.V.
2001 Molecular Biology Problem Solver: A Laboratory Guide Bonventre, J., Walsh, P.and Robinson, D.
2001 The sequence of the human genome Science Venter, J.C., Roberts, al.
2001 The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI. Nucl.Acids Res. Higgins, L.S., Besnier, C.and Kong H.
2001 Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein. J.Biol.Chem. Ghosh, I., Sun, L.and Xu, M.Q.
2001 The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability J. Gen. Virol. Dorsch, S., et al.
2001 The Energetics of the Interaction of BamHI Endonuclease with its Recognition Site GGATCC. J.Mol.Biol. Engler, L.E., Sapienza, P., Dorner, L.F., Kucera, R., Schildkraut, I.and Jen-Jacobson, L.
2001 REBASE-restriction enzymes and methylases. Nucl.Acids Res. Roberts, R.J., and Macelis, D.
2001 Qualitative detection of apoptotic cells assessed by DNA fragmentation. Methods Mol.Biol. McIlwrath, A.J.
2001 Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis. Structure van der Woerd, M.J., Pelletier, J.J., Xu, S.and Friedman, A.M.
2001 Recombinant human DNA (cytosine-5) methyltransferase.III.Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGGCCG sequence. J.Biol.Chem. Bacolla, A., Pradhan, S., Larson, J.E., Roberts, R.J.and Wells, R.D.
2001 Construction of high-complexity combinatorial phage display peptide libraries. Methods Noren, K.A.and Noren, C.J.
2001 Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling Mol Cell. Wu, J.W., et al.
2001 Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene Zhang, A., Gonzales, S.M., Cantor, E.J.and Chong, S.
2001 Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state. EMBO Reports Besnier, C.E.and Kong, H.
2001 Comparative genomics of the restriction-modification systems in Helicobacter pylori. Proc.Natl.Acad.Sci.USA Lin, L.F., Posfai, J., Roberts, R.J.and Kong, H.
2001 Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng. Kalidas, C., Joshi, L., Batt, C.
2001 Hyperthermophilic Inteins. Methods Enzymol. Perler, F.B.
2001 Information access.Building a "GenBank" of the published literature. Science Roberts, R.J., Varmus, H.D., Ashburner, M., Brown, P.O., Eisen, M.B., Khosla, C., Kirschner, M., Nusse, R., Scott, M.and Wold, B.
2001 Hybridization of High-Denisty Filter Arrays of a Brugia malayi BAC Library with Biotinylated Oligonucleotides and PCR Products. BioTechniques Foster, J.M., Kamal, I.H., Daub, J., Swan, M.C., Ingram, J.R., Ganatra, M., Ware, J., Guiliano, D., Aboobaker, A., Moran, L., Blaxter, M.and Slatko, B.E.
2001 Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene Chen, L., Pradhan, S.and Evans, T.C.Jr.
2001 In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore Proc. Natl. Acad. Sci. USA Hanzawa, H., et al.
2001 Determination of Wolbachia Genome Size by Pulsed-Field Gel Electrophoresis. J.Bacteriol. Sun, L.V., Foster, J.M., Tzertzinis, G., Ono, M., Bandi, C., Slatko, B.E.and O'Neill, S.L.
2001 Detection of Serum IgG Antibodies Specific for Wolbachia Surface Protein in Rhesus Monkeys Infected with Brugia malayi. J Infectious Diseases Punkosdy, G.A., Dennis, V.A., Lasater, B.L., Tzertzinis, G.foster, J.M.and Lammie, P.J.
2001 Engineering a Nicking Endonuclease N.AlwI by Domain Swapping Proc.Natl.Acad.Sci.USA Xu, Y., Lunnen, K.and Kong, H.
2001 Discovery and distribution of super-integrons among pseudomonads. Mol.Microbiol. Vaisvila, R., Morgan, R.D., Posfai, J.and Raleigh, E.A.
2001 Restriction endonucleases. In: Molecular biology problem solver Wiley-Liss, Inc. Robinson, D., Walsh, P.R., Bonventre, J.A.
2001 The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA J. Mol. Biol. Fatemi, M., Hermann, A., Pradhan, S., Jeltsch, A.
2002 Acyclic and dideoxy teminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases. Nuc.Acids Res. Gardner, A.F.and Jack, W.E.
2001 Structural study of restriction enzyme MspI/DNA complex by x-ray crystallography Biophys. J. Xu, Q., O'Loughlin, T.J., Kucera, R.B., Schildkraut, I., Guo, H.-C.
2002 A novel cyclophilin from parasitic and free-living nematodes with a unique substrate-and drug-binding domain. J.Biol.Chem. Ma, D., Nelson, L.S., LeCoz, K., Poole, C.and Carlow, C.K.S.
2002 Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity Protein Sci Wojciechowski CL, Cardia JP, and Kantrowitz ER.
2002 Merozoite surface protein-9 of Plasmodium vivax and related simian malaria parasites is orthologous to p101/ABRA of P.falciparum. Mol.Biochem.Parasitol. Vargas-Serrato, E., Barnwell, J.W., Ingravallo, P., Perler, F.B.and Galinski, M.R.
2002 Mechanistic and kinetic considerations of protein splicing. Chem.Rev. Evans, T.C., Jr.and Xu, M-Q
2002 Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein. J.Bacteriol. Southworth, M.W.and Perler, F.B.
2002 Modulation of postendocytic sorting of G protein-coupled receptors Science Whistler, J.L., Enquist, J., Marley, A., Fong, J., Gladher, F., Tsuruda, P., Murray, S.R., Von Zastrow, M.
2002 InBase, the Intein Database. Nuc.Acids Res. Perler, F.B.
2002 Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis J. Bacteriol Nakano, S., et al.
2002 Helminth genomics: from gene discovery to genome sequencing. Trends Parasitol Foster, J.M.and Johnston, D.A.
2002 Inhibition of poly(ADP-ribosyl)ation induces DNA hypermethylation: a possible molecular mechanism. FASEB J. Zardo, G., Reale, A., Passananti, C., Pradhan, S., Bountempo, S., DeMatteis, G., Adams, R.L.P.and Caiafa, P.
2002 Inteins in microbial genomes: distribution, mechanism and function. Scientific World Journal 2 Southworth, M.W.and Perler, F.B.
2002 Intein-mediated affinity-fusion purification of the Escherichia coliRecA protein Protein Expr. Purif.  Singleton, S. F., et al.
2002 Structure of a tRNA Repair Enzyme and Molecular Biology Workhorse: T4 Polynucleotide Kinase. Structure Galburt, E.A., Pelletier, J., Wilson, G.and Stoddard, B.L.
2002 Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi. Int.J.Parasitol. Ware, J., Moran, L., Foster, J., Posfai, J., Vincze, T., Guiliano, D., Blaxter, M., Eisen, J.and Slatko, B.
2002 The mechanism of protein splicing: variations on a theme. Peptides: Proceedings of the Twnety-Seventh European Peptide Symposium Perler, F.B., Chen, L.and Southworth, M.W.
2002 Towards the generation of artificial O6-alkylguanine-DNA alkyltransferases: in vitro selection of antibodies with reactive cysteine residues ChemBioChem Damoiseaux, R. et al
2002 The retinoblastoma gene product interacts with maintenance human DNA (Cytosine-5) methyltransferase and modulates its activity. EMBO J. Pradhan, S.and Kim, G.-D.
2002 Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization Anal Chem. Verhaegen, M. and Christopoulos, T.K.
2002 Purification and initial crystallization studies of a DnaB intein from Synechocystis sp.PCC 6803. Acta Crystallogr.D.Biol.Crystallogr. Chen, X., Xu, M.-Q., Ding, Y., Ferrandon, S.and Rao, Z.
2002 Recombinant Gaussia luciferase. Overexpression, purification and analytical application of a bioluminescent reporter for DNA hybridization Anal Chem Verhaegen, M., Christopoulos, T.K.
2002 Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease. Proc.Natl.Acad.Sci.USA Bitinaite, J.and Schildkraut, I.
2002 Selection for mutants improving expression of an anti-MAP kinase monoclonal antibody by filamentous phage display. J.Immunol.Methods Tuckey, C.D.and Noren, C.J.
2002 Genomic functional annotation using co-evolution profiles of gene clusters. Genome Biol. Zheng, Y., Roberts, R.J.and Kasif, S.
2002 Cloning, Expression and Immunization of the new antigen gene Sj-Ts4 of Schistosoma japonicum. Acta Biochimica et Biophysica Sinica Zhou, D.-M., Yi, X.-Y., Zeng, X.-F., Wang, M., Cai, C., Wang, Q.-L., Zhang, S.-K., McReynolds, L.
2002 Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair Proc. Natl. Acad. Sci. US Moolenaar GF, et al.
2002 Computational Identification of Operons in Microbial Genomes. Genome Res. Zheng, Y., Szustakowski, J.D., Fortnow, L., Roberts, R.J.and Kasif, S.
2002 Creation of an Artificial Bifunctional Intein by Grafting a Homing Endonuclease into a Mini-intein. J.Mol.Biol. Hall, M.F., Noren, C., Perler, F.and Schildkraut, I.
2002 Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases. EMBO J. Kim, G.D., Ni, J., Kelesoglu, N., Roberts, R.J.and Pradhan, S.
2002 Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR Proc. Natl. Acad. Sci. USA Camarero, J.A., et al.
2002 Arm-site binding by lambda -integrase: solution structure and functional characterization of its amino-terminal domain Proc. Natl. Acad. Sci. USA Wojciak, J.M., et al.
2002 Endosomal localization and function of sorting Proc. Natl. Acad. Sci. USA Zhong, Q., et al.
2002 Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR. Nucl.Acids.Res. Wei, A., Yuan, A., Fawcett, G., Butler, A., Davis, T., Xu, S.Y.and Salkoff, L.
2002 Gene therapy: reality or myth for the global bleeding disorders community? Haemophilia Kelley, K., Verma, I.and Pierce, G.F.
2002 Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag Protein Expr. Purif.  Humphries, H. E., Christodoulides, M. and Heckels, J. E.
2002 Development of a Positive Genetic Selection System for Inhibition of Protein Splicing Using Mycobacterial Inteins in Escherichia coli DNA Gyrase Subunit A. J.Mol.Micro.Biotechnol. Adam, E.and Perler, F.B.
2002 Functional analysis of iceA1, a CATG-recognizing restriction endonuclease gene in Helicobacter pylori. Nucl.Acids Res. Xu, Q., Morgan, R.D., Roberts, R.J., Xu, S.Y., van Doorn, L.J., Donahue, J.P., Miller, G.G.and Blaser, M.J.
2002 Crystallographic study of a naturally occurring trans-splicing intein from Synechocystis sp.PCC 6803. Acta Crystallogr.D.Biol.Crystallogr. Chen, X., Xu, M.-Q., Ding, Y., Ferrandon, S.and Rao, Z.
2002 Directed Evolution of Restriction Endonuclease BstYI to Achieve Increased Substrate Specificity. J.Mol.Biol. Samuelson, J.C.and Xu, S.Y.
2002 DNA Binding and Recognition by the IIs Restriction Endonuclease MboII. J.Biol.Chem. Soundararajan, M., Chang, Z., Morgan, R.D., Heslop, P.and Connolly, B.A.
2002 Dirofilaria immitis encodes Di-nhr-7, a putative orthologue of the Drosophila ecdysone-regulated E78 gene. Mol.Biochem.Parasitol. Crossgrove, K., Laudet, V.and Maina, C.V.
2002 Evolutionary relationship of Alw261, Eco31I and Esp3I, restriction endonucleases that recognise overlapping sequences Mol. Genet. Genomics Bitinaite, J., Mitkaite, G., Dauksaite, V., Jakubauskas, A., Timinskas, A., Vaisvila, R., Lubys, A., Janulaitis, A.
2003 A Dual Role for an Aspartic Acid in Glycosylasparaginase Autoproteolysis. Structure Qian, X., Guan, C.and Guo, H.-C.
2003 A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein Proc. Natl. Acad. Sci. USA Hong, S.H., and Maret, W.
2003 Phosphorylation of eIF4E attenuates its interaction with mRNA 5' cap analogs by electrostatic repulsion: intein-mediated protein ligation strategy to obtain phosphorylated protein RNA Zuberek, J., et al.
2003 NEBcutter: A program to cleave DNA with restriction enzymes. Nucl.Acids Res. Vincze, T., Posfai, J.and Roberts, R.J.
2003 Protein trans-splicing in transgenic plant chloroplast: Reconstruction of herbicide resistance from split genes. Proc.Natl.Acad.Sci.USA Chin, H.G., Kim, G.D., Marin, I., Mersha, F., Evans, T.C.Jr., Chen, L., Xu, M.-Q.and Pradhan, S.
2003 Purification and characterisation of a novel DNA methyltransferase, M.AhdI Nucleic Acids Research Marks, P., McGeehan, J., Wilson, G., Errington, N. and Kneale, G.
2003 PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic restriction enzymes. J.Mol.Biol. Pingoud, V., Conzelmann, C., Kinzebach, S., Sudina, A., Metelev, V., Kubareva, E., Bujnicki, J.M., Lurz, R., Luder, G., Xu, S.Y.and Pingoud, A
2003 Kinetic analysis of the coordinated interaction of SgrAI restriction endonuclease with different DNA targets. J.Biol.Chem. Hingorani, K.and Bitinaite, J.
2003 Isolation of BsoBI restriction endonuclease variants with altered substrate specificity. J.Mol.Biol. Zhu, Z., Zhou, J., Friedman, A.M.and Xu, S.-Y.
2003 Mammalian DNA (cytosine-5) methyltransferases and their expression. Clin.Immunol. Pradhan, S.and Esteve P.-O.
2003 Merging fluorescence resonance energy transfer and expressed protein ligation to analyze protein-protein interactions Anal. Biochem.  Scheibner, K.A., Zhang, Z. and Cole, P.A.
2003 Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis J. Biol. Chem. Wuebbens, M. M. and Rajagopalan, K. V.
2003 Purification and characterization of the beta-trefoil fold protein barley alpha-amylase/subtilisin inhibitor overexpressed in Escherichia coli Protein Expr. Purif. Bonsager, B.C., et al.
2003 Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions. Biotechnol.Prog. Sharma, S., Zhang, A., Wang, H., Harcum, S.W.and Chong S.
2003 Structure of the large FK506-binding protein FKBP51, an Hsp90-binding protein and a component of steroid receptor complexes Proc. Natl. Acad. Sci. USA Sinars, C.R., et al.
2003 The role of C-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation J. Biol. Chem. Zhang, Z., et al.
2003 Zinc Ion Effects on Individual Ssp DnaE Intein Splicing Steps: Regulating Pathway Progression. Biochemistry Nichols, N.M., Benner, J.S., Martin, D.D.and Evans, T.C.Jr.
2003 Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex. Proc.Natl.Acad.Sci.USA Wang, H.and Chong, S.
2003 REBASE Restriction enzymes and methyltransferases. Nucl.Acids Res. Roberts, R.J., Vincze, T., Posfai, J.and Macelis, D.