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We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,000 publications (as of 1/17) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
2001 AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucl.Acids Res. Cheng, X.and Roberts, R.J.
2001 Characterization of a naturally occurring trans-splicing intein from Synechocystis sp.PCC6803. Biochemistry Martin, D.D., Xu, M-Q and Evans, T.C.Jr.
2001 Cell-free translation reconstituted with purified components Nat. Biotech. Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K. and Ueda, T.
2001 Protein trans-Splicing To Produce Herbicide-Resistant Acetolactate Synthase. Appl.Environ.Microbiol. Sun, L., Ghosh, I., Paulus, H.and Xu, M.Q.
2001 Protein splicing and its aplications. Genet.Eng.(NY) Giriat, I., Muir, T.W.and Perler, F.B.
2001 Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. BioTechniques Southworth, M.W., Amaya, K., Evans, T.C., Xu, M-Q and Perler, F.B.
2001 PubMed Central: The GenBank of the Published Literature. Proc.Natl.Acad.Sci. Roberts, R.J.
2001 Protein splicing. Encyclopedia of Genetics Xu, M.Q., Chong, S.and T.C.Evans, Jr.
2001 Intein-mediated rapid purification of cre recombinase Protein Expr.Purif. Cantor, E.J.and Chong, S.
2001 Intein-Mediated Ligation and Cyclization of Expressed Proteins. Methods Xu, M.Q.and T.C.Evans, Jr.
2001 Nuclear receptors in nematodes: themes and variations. Trends in Genetics Sluder, A.E.and Maina, C.V.
2001 Molecular Biology Problem Solver: A Laboratory Guide Bonventre, J., Walsh, P.and Robinson, D.
2001 The sequence of the human genome Science Venter, J.C., Roberts, R.J.et al.
2001 The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI. Nucl.Acids Res. Higgins, L.S., Besnier, C.and Kong H.
2001 Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein. J.Biol.Chem. Ghosh, I., Sun, L.and Xu, M.Q.
2001 The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability J. Gen. Virol. Dorsch, S., et al.
2001 REBASE-restriction enzymes and methylases. Nucl.Acids Res. Roberts, R.J., and Macelis, D.
2001 The Energetics of the Interaction of BamHI Endonuclease with its Recognition Site GGATCC. J.Mol.Biol. Engler, L.E., Sapienza, P., Dorner, L.F., Kucera, R., Schildkraut, I.and Jen-Jacobson, L.
2001 Qualitative detection of apoptotic cells assessed by DNA fragmentation. Methods Mol.Biol. McIlwrath, A.J.
2001 Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis. Structure van der Woerd, M.J., Pelletier, J.J., Xu, S.and Friedman, A.M.
2001 Recombinant human DNA (cytosine-5) methyltransferase.III.Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGGCCG sequence. J.Biol.Chem. Bacolla, A., Pradhan, S., Larson, J.E., Roberts, R.J.and Wells, R.D.
2001 Construction of high-complexity combinatorial phage display peptide libraries. Methods Noren, K.A.and Noren, C.J.
2001 Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene Zhang, A., Gonzales, S.M., Cantor, E.J.and Chong, S.
2001 Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling Mol Cell. Wu, J.W., et al.
2001 Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state. EMBO Reports Besnier, C.E.and Kong, H.
2001 Comparative genomics of the restriction-modification systems in Helicobacter pylori. Proc.Natl.Acad.Sci.USA Lin, L.F., Posfai, J., Roberts, R.J.and Kong, H.
2001 Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng. Kalidas, C., Joshi, L., Batt, C.
2001 Hyperthermophilic Inteins. Methods Enzymol. Perler, F.B.
2001 Hybridization of High-Denisty Filter Arrays of a Brugia malayi BAC Library with Biotinylated Oligonucleotides and PCR Products. BioTechniques Foster, J.M., Kamal, I.H., Daub, J., Swan, M.C., Ingram, J.R., Ganatra, M., Ware, J., Guiliano, D., Aboobaker, A., Moran, L., Blaxter, M.and Slatko, B.E.
2001 Information access.Building a "GenBank" of the published literature. Science Roberts, R.J., Varmus, H.D., Ashburner, M., Brown, P.O., Eisen, M.B., Khosla, C., Kirschner, M., Nusse, R., Scott, M.and Wold, B.
2001 In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore Proc. Natl. Acad. Sci. USA Hanzawa, H., et al.
2001 Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene Chen, L., Pradhan, S.and Evans, T.C.Jr.
2001 Determination of Wolbachia Genome Size by Pulsed-Field Gel Electrophoresis. J.Bacteriol. Sun, L.V., Foster, J.M., Tzertzinis, G., Ono, M., Bandi, C., Slatko, B.E.and O'Neill, S.L.
2001 Detection of Serum IgG Antibodies Specific for Wolbachia Surface Protein in Rhesus Monkeys Infected with Brugia malayi. J Infectious Diseases Punkosdy, G.A., Dennis, V.A., Lasater, B.L., Tzertzinis, G.foster, J.M.and Lammie, P.J.
2001 Engineering a Nicking Endonuclease N.AlwI by Domain Swapping Proc.Natl.Acad.Sci.USA Xu, Y., Lunnen, K.and Kong, H.
2001 Discovery and distribution of super-integrons among pseudomonads. Mol.Microbiol. Vaisvila, R., Morgan, R.D., Posfai, J.and Raleigh, E.A.
2001 Restriction endonucleases. In: Molecular biology problem solver Wiley-Liss, Inc. Robinson, D., Walsh, P.R., Bonventre, J.A.
2001 The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA J. Mol. Biol. Fatemi, M., Hermann, A., Pradhan, S., Jeltsch, A.
2001 Structural study of restriction enzyme MspI/DNA complex by x-ray crystallography Biophys. J. Xu, Q., O'Loughlin, T.J., Kucera, R.B., Schildkraut, I., Guo, H.-C.
2011 Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae Nucleic Acids Res. Midon, M., Schäfer, P., Pingoud, A., Ghosh, M., Moon, A.F., Cuneo, M.J., London, R.E. and Meiss, G.
2011 Kinetic study of de novo chromophore maturation of fluorescent proteins Anal. Biochem. Iizuka, R., Yamagishi-Shirasaki, M. and Funatsu, T.
2011 Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity. Nucl.Acids.Res. Chan, S.H., Stoddard, B.L.and Xu, S.Y.
2011 Intravesicle Isothermal DNA Replication. BMC Res Notes Torino, D., Del Bianco, C., Ross, L., Ong, J.and Mansy, S.
2011 Introduction and historical overview of DNA sequencing Curr. Prot. Mol. Biol. Nelson, F.K, et. al.
2011 Kinetic characterization of single strand break ligation in duplex DNA by T4 DNA Ligase. J Biol Chem. Lohman, G.J.S., Chen, L.and Evans, T.C., Jr.
2011 Ordered and dynamic assembly of single spliceoseoms Science Hoskins, A. et al.
2011 Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconvential use of oxidizing environments with a maltose binding protein-hexahistidine dual tag to enhance membrane protein yield Microbial Cell Factories Tait, A.R. and Straus, S.K.
2011 Overview of DNA Sequencing Strategies Curr.Protoc.Mol.Biol. Shendure, J.A., Porreca, G.J., Church, G.M., Gardner, A.F.Hendrickson, C.L., Kieleczawa, J.and Slatko, B.E.
2011 New Clues in the Allosteric Activation of DNA Cleavage by SgrAI; Structures of SgrAI Bound to Cleaved Primary Site DNA and Uncleaved Secondary Site DNA. Acta Crystallogr.F.Structural biology and crystallization communications Little, E.J., Dunten, P.W., Bitinaite, J.and Horton, N.C.
2011 Next-gen expression systems Ritter, A.
2011 Optimization of Medium Composition for Production of Recombinant Calf Chymosin from Kluyveromyces lactis in Submerged Fermentation J. of Northeast Agricultural University Feng, Z., et al.
2011 Identification of targeting peptides for ischemic myocardium by in vivo phage display J. Mol. Cell. Cardiol. Kanki, S. et al.
2011 Genome-wide mapping of the sixth base Genome Biol. Diep, D. and Zhang, K.
2011 Heterologous expression of a thermophilic esterase in Kluyveromyces yeasts Appl. Microbiol. Biotechnol. Rocha S.N., et al.
2011 High sensitivity 4-hydroxymethylcytosine detection in Balb/C brain tissue. J Vis Exp. Davis, T.and Vaisvila, R.
2011 "First Generation" automated DNA sequencing technology Curr. Prot. Mol. Biol. Slatko, B.E., et al.
2011 Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase Methods Mol.Biol. Zhang, A., Sun, L., Buswell, J., Considine, N., Ghosh, I., Masharina, A., Noren, C.and Xu, M.Q.
2011 Fluorescent peptides highlight peripheral nerves during surgery in mice Nat. Biotechnol. Whitney, M.A. et al.
2011 Identification and characterization of protein glycosylation using specific endo- and exoglycosidases. Methods Mol.Biol. Magnelli, P., Bielik, A.and Guthrie, E.
2011 Identification and characterization of the cofactor-independent phosphoglycerate mutases of Dirofilaria immitis and its Wolbachia endosymbiont. Veterinary Parasitology Li.Z., Galvin, B., Raverdy, S.and Carlow, C.K.S.
2011 Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae J Bacteriol. Yamamoto, S., Izumiya, H., Mitobe, J., Morita, M., Arakawa, E., Ohnishi, M. and Watanabe, H.
2011 High-throughput analysis of the mutagenic and cytotoxic properties of DNA lesions by next-generation sequencing Nuc. Acids Res. Yuan, B., Wang, J., Cao, H. et al
2011 Hypomethylation of a LINE-1 Promoter Activates an Alternate Transcript of the MET Oncogene in Bladders with Cancer PLoS Genet. Wolff, E.M. et al. Epigenetics
2011 Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases J Vis Exp. Magnelli PE, Bielik AM, Guthrie EP.
2011 Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2011-31 May 2011. Mol Ecol Resour. Molecular Ecology Resources Primer Development Consortium, Bonizzoni, M., Bourjea, J., Chen, B., Crain, B.J., Cui, L., Fiorentino, V., Hartmann, S., Hendricks, S., Ketmaier, V., Ma, X., Muths, D., Pavesi, L., Pfautsch, S., Rieger, M.A., Santonastaso, T., Sattabongkot, J., Taron, C.H., Taron, D.J., Tiedemann, R., Yan, G., Zheng, B.and Zhong, D.
2011 The MspJI family of modification-dependent restriction endonucleases for epigenetic studies. Proc.Natl.Acad.Sci. Cohen-Karni, D., Xu, D., Apone, L., Formenkov, A., Sun, Z., Davis, P.J., Morey Kinney, S.R., Yamada-Mabuchi, M., Xu, S.-y., Davis, T., Pradhan, S.Roberts, R.J., Zheng, Y.
2011 Tissue specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genome J. Biol. Chem. Kinney, S.M. et al.
2011 TrbB from conjugative plasmid F is a structurally distinct disulfide isomerase that requires DsbD for redox state maintenance. J Bacteriol Hemmis, C.W., Berkmen, M., Eser, M.and Schildbach, J.F.
2011 Recent developments in difficult protein expression: A guide to E. colistrains, promoters, and relevant host mutations. Methods in Molecular Biology Samuelson, J.C.
2011 "The Molecular Biology, Immunology and chemotherapy of Wolbachia bacterial endosymbionts of filarial nematodes". Parasitic Nematodes: Molecular Biology, Biochemistry & Immunology (Kennedy, M.and Harnett, W., eds.) Foster, J.M., Hoerauf, A., Slatko, B.E.and Taylor, M.J.
2011 The MspJI family of modification-dependent restriction endonucleases for epigenetic studies Proc. Natl. Acad. Sci. Cohen-Karni, D, et al.
2011 Uptake of radiolabeled GlcNAc into Saccharomyces cerevisiae via native hexose transporters and its in vivo incorporation into GPI precursors in cells expressing heterologous GlcNAc kinase. REMS Yeast Res Scarcelli, J.J., Colussi, P.A., Fabre, A.L., Boles, E., Orlean, P.and Taron, C.H.
2011 Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants Nucleic Acids Res. De Masi, F., Grove, C.A., Vedenko, A., Alibés, A., Gisselbrecht, S.S., Serrano, L., Bulyk, M.L. and Walhout, A.J.
2011 Tuned Escherichia coli as a host for the expression of disulfide-rich proteins Biotechnology Journal Salinas, G., Pellizza, L., Margenat, M., et al.
2011 YaeJ is a novel ribosome-associated protein inEscherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes Nucleic Acids Res.  Handa, Y., Inaho, N. and Nameki, N.
2011 Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening Biotechnology Journal Correa, A. and Oppezzo, P.
2011 Two Color STED microscopy in living cells. Biomedical Optics Express Pellett, P.A., Sun, X., Gould, T.J., Rothman, J.E., Xu, M-Q, Corrêa, I.R.Jr.and Bewersdorf, J.
2011 Regulation of expression and activity of DNA (cytosine-5) methyltransferases in mammalian cells Prog Mol Biol Transl Sci. Kinney, S.R. and Pradhan, S.
2011 The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes Biochem J.  Panayiotou, C., Solaroli, N., Xu, Y., Johansson, M. and Karlsson, A.
2011 Screening of specific binding peptide targeting blood vessel of human esophageal cancer in vivo in mice Chin. Med. J. Zhi, M. et al.
2011 Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries Molecules Lamichhane, T.N., Abeydeera, N.D., Duc, A.C., Cunningham, P.R. and Chow, C.S
2011 Potent Anti-Tumor Effect Generated by a Novel Human Papilllomavirus (HPV) Antagonist Peptide Reactivating the pRb/E2F Pathway PLoS One Guo, C.P. et al.
2011 "Recent Developments in Difficult Protein Expression: A Guide to E.coli strains, Promoters and Relevant Host Mutations" editors Tom C.Evans Jr.and Ming-Qun Xu, Humana Press Methods Mol.Biol. Samuelson, J.C.
2011 Recombination in Wolbachia endosymbionts of filarial nematodies? Appl.Environ.Microbiol. Foster, J., Slatko, B., Bandi, C.and Kumar, S.
2011 Targeting choroid plexus epithelia and ventricular ependyma for drug delivery to the central nervous system BMC Neurosci Gonzalez, A.M. et al. Phage Display
2011 Targeting the Wolbachia Cell Division Protein FtsZ as a New Approach for Antifilarial Therapy. PLoS Neglected Tropical Diseases Li, Z., Garner, A.L., Gloeckner, C., Janda, K.D.and Carlow, C.K.
2011 The Arthrobacter Species FB24 Arth_1007 (DnaB) Intein Is a Pseudogene. PLoS ONE Tori, K.and Perler, F.B.
2011 Simple and efficient synthesis of 5′ pre-adenylated DNA using thermostable RNA ligase. Nucl.Acids Res. Zhelkovsky, A.M.and McReynolds, L.A.
2011 Site-specific protein labeling by intein-mediated protein ligation. Methods Mol.Biol. Ghosh, I., Considine, N., Maunus, E., Sun, L., Zhang, A., Buswell, J., Evans, T.C.Jr.and Xu, M.Q.
2011 T4 RNA ligase 2 truncated active site mutants: improved tools for RNA analysis. BMC Biotechnol. Viollet, S., Fuchs, R.T., Munafo, D.B., Zhuang, F.and Robb, G.B.
2011 Activity, specificity and structure of I-Bth03051: a representative of a new homing endonuclease family Taylor, G.K., Heiter, D.F., Pietrokovski, S. and Stoddard, B.L.
2011 Asymmetric DNA recognition by the OkrAI endonuclease, an isoschizomer of BamHI. Nucl.Acids.Res. Vanamee, E.S., Viadiu, H., Chan, S.H., Ummat, A., Hartline, A.M., Xu, S.Y.and Aggarwal, A.K.
2011 Biochemical Characterization of a Structure-Specific Resolving Enzyme from Sulfolobus Islandicus Rod-Shaped Virus 2. PLoS ONE Gardner, A.F., Guan, C.and Jack, W.E.
2011 A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis. FEMS Yeast Res. Ganatra, M.B., Vainauskus, S., Hong, J.M., Taylor, T.E., Denson, J.P., Esposito, D., Read, J.D., Schmeisser, H., Zoon, K.C., Hartley, J.L.and Taron, C.H.
2011 A SNAP-tagged detivative of HIV-1 - A versatile tool to study virus-cell interactions PLoS One Eckhardt, M. et al.
2011 A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp.aureus USA300. Nucl.Acids.Res. Xu, S.Y., Corvaglia, A.R., Chan, S.H., Zheng, Y.and Linder, P.
2011 Characterization of MspNI (G/GWCC) and MspNII (R/GATCY), novel thermostable Type II restriction endonucleases from Meiothermus sp., isoschizomers of AvaII and BstYI Mol Biol Rep. Gupta, R., Xu, S.Y., Sharma, P. and Capalash, N.
2011 Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq Genome Res. Saiful, I., Kjällquist, U., Moliner, A. et al
2011 Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonuclease. Extremophiles Gardner, A.F., Prangishvili, D.and Jack.W.E.
2011 Branched Intermediate Formation is the Slowest Step in th Protein Splicing Reaction of the Ala1 KbA Intein from Methanococcus jannaschii Saleh, L., Southworth, M.W., Considine, N., O'Neill, C., Benner, J., Bollinger, J.M. and Perler, F.B.