We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,000 publications (as of 1/17) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
2001 AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucl.Acids Res. Cheng, X.and Roberts, R.J.
2001 Characterization of a naturally occurring trans-splicing intein from Synechocystis sp.PCC6803. Biochemistry Martin, D.D., Xu, M-Q and Evans, T.C.Jr.
2001 Cell-free translation reconstituted with purified components Nat. Biotech. Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K. and Ueda, T.
2001 Protein trans-Splicing To Produce Herbicide-Resistant Acetolactate Synthase. Appl.Environ.Microbiol. Sun, L., Ghosh, I., Paulus, H.and Xu, M.Q.
2001 Protein splicing and its aplications. Genet.Eng.(NY) Giriat, I., Muir, T.W.and Perler, F.B.
2001 Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. BioTechniques Southworth, M.W., Amaya, K., Evans, T.C., Xu, M-Q and Perler, F.B.
2001 PubMed Central: The GenBank of the Published Literature. Proc.Natl.Acad.Sci. Roberts, R.J.
2001 Protein splicing. Encyclopedia of Genetics Xu, M.Q., Chong, S.and T.C.Evans, Jr.
2001 Intein-mediated rapid purification of cre recombinase Protein Expr.Purif. Cantor, E.J.and Chong, S.
2001 Intein-Mediated Ligation and Cyclization of Expressed Proteins. Methods Xu, M.Q.and T.C.Evans, Jr.
2001 Nuclear receptors in nematodes: themes and variations. Trends in Genetics Sluder, A.E.and Maina, C.V.
2001 Molecular Biology Problem Solver: A Laboratory Guide Bonventre, J., Walsh, P.and Robinson, D.
2001 The sequence of the human genome Science Venter, J.C., Roberts, al.
2001 The nicking endonuclease N.BstNBI is closely related to Type IIs restriction endonucleases MlyI and PleI. Nucl.Acids Res. Higgins, L.S., Besnier, C.and Kong H.
2001 Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein. J.Biol.Chem. Ghosh, I., Sun, L.and Xu, M.Q.
2001 The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability J. Gen. Virol. Dorsch, S., et al.
2001 The Energetics of the Interaction of BamHI Endonuclease with its Recognition Site GGATCC. J.Mol.Biol. Engler, L.E., Sapienza, P., Dorner, L.F., Kucera, R., Schildkraut, I.and Jen-Jacobson, L.
2001 REBASE-restriction enzymes and methylases. Nucl.Acids Res. Roberts, R.J., and Macelis, D.
2001 Qualitative detection of apoptotic cells assessed by DNA fragmentation. Methods Mol.Biol. McIlwrath, A.J.
2001 Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis. Structure van der Woerd, M.J., Pelletier, J.J., Xu, S.and Friedman, A.M.
2001 Recombinant human DNA (cytosine-5) methyltransferase.III.Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGGCCG sequence. J.Biol.Chem. Bacolla, A., Pradhan, S., Larson, J.E., Roberts, R.J.and Wells, R.D.
2001 Construction of high-complexity combinatorial phage display peptide libraries. Methods Noren, K.A.and Noren, C.J.
2001 Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene Zhang, A., Gonzales, S.M., Cantor, E.J.and Chong, S.
2001 Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling Mol Cell. Wu, J.W., et al.
2001 Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state. EMBO Reports Besnier, C.E.and Kong, H.
2001 Comparative genomics of the restriction-modification systems in Helicobacter pylori. Proc.Natl.Acad.Sci.USA Lin, L.F., Posfai, J., Roberts, R.J.and Kong, H.
2001 Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris Protein Eng. Kalidas, C., Joshi, L., Batt, C.
2001 Hyperthermophilic Inteins. Methods Enzymol. Perler, F.B.
2001 Hybridization of High-Denisty Filter Arrays of a Brugia malayi BAC Library with Biotinylated Oligonucleotides and PCR Products. BioTechniques Foster, J.M., Kamal, I.H., Daub, J., Swan, M.C., Ingram, J.R., Ganatra, M., Ware, J., Guiliano, D., Aboobaker, A., Moran, L., Blaxter, M.and Slatko, B.E.
2001 Information access.Building a "GenBank" of the published literature. Science Roberts, R.J., Varmus, H.D., Ashburner, M., Brown, P.O., Eisen, M.B., Khosla, C., Kirschner, M., Nusse, R., Scott, M.and Wold, B.
2001 In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore Proc. Natl. Acad. Sci. USA Hanzawa, H., et al.
2001 Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene Chen, L., Pradhan, S.and Evans, T.C.Jr.
2001 Determination of Wolbachia Genome Size by Pulsed-Field Gel Electrophoresis. J.Bacteriol. Sun, L.V., Foster, J.M., Tzertzinis, G., Ono, M., Bandi, C., Slatko, B.E.and O'Neill, S.L.
2001 Detection of Serum IgG Antibodies Specific for Wolbachia Surface Protein in Rhesus Monkeys Infected with Brugia malayi. J Infectious Diseases Punkosdy, G.A., Dennis, V.A., Lasater, B.L., Tzertzinis, G.foster, J.M.and Lammie, P.J.
2001 Engineering a Nicking Endonuclease N.AlwI by Domain Swapping Proc.Natl.Acad.Sci.USA Xu, Y., Lunnen, K.and Kong, H.
2001 Discovery and distribution of super-integrons among pseudomonads. Mol.Microbiol. Vaisvila, R., Morgan, R.D., Posfai, J.and Raleigh, E.A.
2001 Restriction endonucleases. In: Molecular biology problem solver Wiley-Liss, Inc. Robinson, D., Walsh, P.R., Bonventre, J.A.
2001 The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA J. Mol. Biol. Fatemi, M., Hermann, A., Pradhan, S., Jeltsch, A.
2001 Structural study of restriction enzyme MspI/DNA complex by x-ray crystallography Biophys. J. Xu, Q., O'Loughlin, T.J., Kucera, R.B., Schildkraut, I., Guo, H.-C.
2003 A Dual Role for an Aspartic Acid in Glycosylasparaginase Autoproteolysis. Structure Qian, X., Guan, C.and Guo, H.-C.
2003 A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein Proc. Natl. Acad. Sci. USA Hong, S.H., and Maret, W.
2003 NEBcutter: A program to cleave DNA with restriction enzymes. Nucl.Acids Res. Vincze, T., Posfai, J.and Roberts, R.J.
2003 Phosphorylation of eIF4E attenuates its interaction with mRNA 5' cap analogs by electrostatic repulsion: intein-mediated protein ligation strategy to obtain phosphorylated protein RNA Zuberek, J., et al.
2003 Protein trans-splicing in transgenic plant chloroplast: Reconstruction of herbicide resistance from split genes. Proc.Natl.Acad.Sci.USA Chin, H.G., Kim, G.D., Marin, I., Mersha, F., Evans, T.C.Jr., Chen, L., Xu, M.-Q.and Pradhan, S.
2003 Purification and characterisation of a novel DNA methyltransferase, M.AhdI Nucleic Acids Research Marks, P., McGeehan, J., Wilson, G., Errington, N. and Kneale, G.
2003 PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic restriction enzymes. J.Mol.Biol. Pingoud, V., Conzelmann, C., Kinzebach, S., Sudina, A., Metelev, V., Kubareva, E., Bujnicki, J.M., Lurz, R., Luder, G., Xu, S.Y.and Pingoud, A
2003 Kinetic analysis of the coordinated interaction of SgrAI restriction endonuclease with different DNA targets. J.Biol.Chem. Hingorani, K.and Bitinaite, J.
2003 Isolation of BsoBI restriction endonuclease variants with altered substrate specificity. J.Mol.Biol. Zhu, Z., Zhou, J., Friedman, A.M.and Xu, S.-Y.
2003 Mammalian DNA (cytosine-5) methyltransferases and their expression. Clin.Immunol. Pradhan, S.and Esteve P.-O.
2003 Merging fluorescence resonance energy transfer and expressed protein ligation to analyze protein-protein interactions Anal. Biochem.  Scheibner, K.A., Zhang, Z. and Cole, P.A.
2003 Purification and characterization of the beta-trefoil fold protein barley alpha-amylase/subtilisin inhibitor overexpressed in Escherichia coli Protein Expr. Purif. Bonsager, B.C., et al.
2003 Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis J. Biol. Chem. Wuebbens, M. M. and Rajagopalan, K. V.
2003 Structure of the large FK506-binding protein FKBP51, an Hsp90-binding protein and a component of steroid receptor complexes Proc. Natl. Acad. Sci. USA Sinars, C.R., et al.
2003 Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions. Biotechnol.Prog. Sharma, S., Zhang, A., Wang, H., Harcum, S.W.and Chong S.
2003 Zinc Ion Effects on Individual Ssp DnaE Intein Splicing Steps: Regulating Pathway Progression. Biochemistry Nichols, N.M., Benner, J.S., Martin, D.D.and Evans, T.C.Jr.
2003 The role of C-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation J. Biol. Chem. Zhang, Z., et al.
2003 Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex. Proc.Natl.Acad.Sci.USA Wang, H.and Chong, S.
2003 REBASE Restriction enzymes and methyltransferases. Nucl.Acids Res. Roberts, R.J., Vincze, T., Posfai, J.and Macelis, D.
2003 Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants. Nucl.Acids Res. Sandman, K.E., Tardiff, D.F., Neely, L.A.and Noren, C.J.
2003 Purification of recombinant proteins from E.coli by engineered inteins. Methods Mol.Biol. Xu, M.-Q.and Evans, T.C., Jr.
2003 Intein-mediated Protein Purification Methods and Tools in Biosciences and Medicine Chong, S.and Perler, F.B.
2003 Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase Science Rak, A., et al.
2003 Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8 Biol. Chem. David, R., et al.
2003 Allosteric Activator Domain of Maintenance Human DNA (Cytosine-5) Methyltransferase and Its Role in Methylation Spreading. Biochemistry Pradhan, S.and Esteve P.-O.
2003 An aspartate aminotransferase of Wolbachia endobacteria from Onchocerca volvulus is recognized by IgG1 antibodies from residents of endemic areas. Parasitol.Res. Fischer, P., Bonow, I., Buttner, D.W., Kamal, I.H.and Liebau, E.
2003 A single surface tryptophan in the chitin-binding domain from Bacillus circulans chitinase A1 plays a pivotal role in binding chitin and can be modified to create an elutable affinity tag. Biochim.Biophys.Acta. Ferrandon, S., Sterzenbach, T., Mersha, F.B.and Xu, M-Q
2003 Assembly of high-affinity insulin receptor agonists and antagonists from peptide building blocks Proc. Natl. Acad. Sci. USA Schaffer, L., et al.
2003 A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucl.Acids Res. Roberts, R.J., Belfort, al.
2003 A general method for the covalent labeling of fusion proteins with small molecules in vivo Nature Biotechnology Keppler A. et al.
2003 Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates Proc. Natl. Acad. Sci. USA Salazar, J.C., et al.
2003 Cloning and Biochemical Characterization of Blisterase, a Subtilisin-like Convertase from the Filarial Parasite, Onchocerca volvulus. J.Biol.Chem. Poole, C.B., Jin, J.and McReynolds, L.A.
2003 Generation of an affinity column for antibody purification by intein-mediated protein ligation. J.Immunol.Methods Sun, L., Ghosh, I.and Xu, M.Q.
2003 A fusion of disciplines: chemical approaches to exploit fusion proteins for functional genomics Chembiochem Johnsson N. and Johnsson K.
2003 DNA -repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a Proc. Natl. Acad. Sci. USA Walters, K.J., et al.
2003 Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process Biochemistry Yee, C.S., et al.
2003 Induced protein dimerizaton in vivo through covalent labeling JACS Gendreizig, S. et al.
2003 Glucocorticoid Receptor-like Zn(Cys)4 Motifs in Bsl I Restriction Endonuclease. J.Mol.Biol. Vanamee, E.S., Hsieh, P.C., Zhu, Z., Yates, D., Garman, E., Xu, S.Y.and Aggarwal, A.K.
2003 Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing. J.Biol.Chem. Ding, Y., Xu, M.-Q., Ghosh, I., Chen, X., Ferrandon, S., Lesage, G., Rao, Z.
2003 Covalent labeling of fusion proteins with chemical probes in living cells Chimia Gendreizig S. et al.
2003 Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo Chem. Biol.  Juillerat A. et al.
2003 Covalent and selective immobilization of fusion proteins JACS Kindermann M. et al.
2003 Cytosine methylation by the SuaI restriction-modification system: implications for genetic fidelity in a hyperthermophilic archaeon. J.Bacteriol. Grogan, D.W.
2003 Crystallization of restriction endonuclease SfiI in complex with DNA Acta Crystallogr. D Biol. Crystallogr. Viadiu, H., Vanamee, E.S., Jacobson, E.M., Schildkraut, I. and Aggarawl, A.K.
2000 An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile. EMBO J. Southworth, M.W., Benner, J.and Perler, F.B.
2000 Characterization of the Specific DNA Nicking Activity of Restriction Endonuclease N.BstNBI. Biol.Chem. Morgan, R., Calvet, C., Demeter, M., Agra, R.and Kong, H.
2000 Cloning, Expression and Purification of a Thermostable Nonhomodimeric Restriction Enzyme, BslI J.Bacteriol. Hsieh, P.-C., Xiao, J.-P., O'Loane, D.and Xu, S.-Y.
2000 Expression and purification of recombinant proteins by fusion to maltose-binding protein. Mol.Biotechnol. Riggs, P.
2000 Fusions to Self-Splicing Inteins for Protein Purification. Methods in Enzymology Xu, M.Q., Paulus, H.and Chong, S.
2000 A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis. Nucl.Acids Res. Biery, M.C., Stewart, F.J., Stellwagen, A.E., Raleigh, E.A.and Craig, N.L.
2000 Functional analysis of putative restriction-modification system genes in the Helicobacter pylori J99 genome. Nucl.Acids Res. Kong, H., Lin, L.F., Porter, N., Stickel, S.Byrd, D., Posfai, J.and Roberts R.J.
2000 Dissecting the Chemistry of Protein Splicing and its Applications Angew.Chem.Int.Ed. Noren, C.J., Wang, J.and Perler, F.B.
2000 Crystal Structure of the Complex of Brugia Malayi Cyclophilin and Cyclosporin A. Protein Sci. Ellis, P.J., Carlow, C.K.S., Ma, D.and Kuhn, P.
2000 Construction and use of pIII-displayed peptide libraries Phage Display: A Laboratory Manual Noren, K.A., Saltman, L.H.and Noren, C.J.
2000 Crystallization and preliminary X-ray diffraction analysis of MspI restriction endonuclease in complex with its cognate DNA. Acta Crystallogr.D.Biol.Crystallogr. O'Loughlin, T.J., Xu, Q., Kucera, R.B., Dorner, L.F., Sweeney, S., Schildkraut, I.and Guo, H.-C.
2000 Crystallization of Restriction Endonuclease BamHI with Nonspecific DNA. J.Struct.Biol. Viadiu, H., Kucera, R., Schildkraut, I.and Aggarwal, A.K.
2000 Hybrid mouse-prokaryotic DNA (Cytosine-5) methyltransferase retain the specificity of the parental C-terminal domain. EMBO J. Pradhan, S and Roberts, R.J.
2000 Identification of the Metal-Binding Sites of Restriction Endonucleases by Fe2+-Mediated Oxidative Cleavage. Biochemistry Hlavaty, J.J., Benner, J.S., Hornstra, L.J.and Schildkraut, I.
2000 Implementation of Automation in a Small-Scale DNA Sequencing Core Facility. J.Biomolecular Techniques Ware, J.L., Moran, L., Lin, C.-L.and Slatko, B.
2000 Identification of type II restriction and modification systems in Helicobacter pylori reveals their substantial diversity among strains. Proc.Natl.Acad.Sci.USA Xu, Q., Morgan, R.D., Roberts, R.J.and Blaser, M.J.
2000 Methyl-specific DNA Binding by McrBC, a Modification-dependent Restriction Enzyme. J.Mol.Biol. Stewart, F.J., Panne, D., Bickle, T.A.and Raleigh, E.A.