Enzymes that manipulate glycans and glycopeptides

A major focus of our research is in the areas of glycobiology and glycomics. In general, we are interested in biological or biotechnological glycobiology problems that can be addressed through discovery of novel enzymes (or binding proteins) and/or improved analytical workflows. Areas of interest have involved improving workflows for analysis of N- and O-glycans, GPI anchors, and glycosphingolipid head groups. Working with many academic and industrial collaborators, we help apply new enzymes or workflows to various topics in glycobiology including glycoprotein analysis, biologic drug quality control, serum glycan biomarker discovery, and parasitology.

 

A schematic depicting N- and O-linked glycosylation on a membrane bound cell surface protein. Sugars are shown in symbolic SNFG nomenclature (green circle, mannose; blue square, N-acetylglucosamine; yellow circle, galactose; red triangle, fucose; yellow square, N-acetylgalactosamine; purple diamond, sialic acid).

 

References:

Vainauskas, S., Guntz, H., McLeod, E., McClung, C., Ruse, C.I., Shi, X. and Taron, C.H. (2021) A broad-specificity O-glycoprotease that enables improved analysis of glycoproteins and glycopeptides containing intact complex O-glycans. Anal. Chem. PMID: 34962767

Ganatra, M.B., Potapov, V., Vainauskas, S., Francis, A.Z., McClung, C.M., Ruse, C.I., Ong, J.L. and Taron, C.H. (2021) A bi-specific lectin from the mushroom Boletopsis grisea and its application in glycoanalytical workflows. Sci. Rep. PMID: 33420304

Vainauskas, S., Kirk, C., Petralia, L., Guthrie, E.P., McLeod, E., Bielik, A., Luebbers, A., Foster, J., Hokke, C.H., Rudd, P.M., Shi, X. and Taron, C.H. (2018) A novel broad specificity fucosidase capable of core α(1,6) fucose release from N-glycans labeled with urea-linked fluorescent dyes. Sci. Rep. PMID: 29934601

Vainauskas, S., Duke, R.M., McFarland, J., McClung, C., Ruse, C. and Taron, C.H. (2016) Profiling of core fucosylated N-glycans using a novel bacterial lectin that specifically recognizes a1,6 fucosylated chitobiose. Sci. Rep. PMID: 27678371

Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. (2016) Comprehensive profiling of glycosphingolipid glycans using a novel broad specificity endoglycoceramidase in a high-throughput workflow. Anal. Chem. PMID: 27033327