Engineering of a thermolabile Proteinase K
Thermolabile Proteinase K is an engineered variant which can be easily heat inactivated. This feature is beneficial for minimizing substrate loss in workflows involving multiple enzymatic steps.
Thermolabile Proteinase K (TLPK) may be heat inactivated in 10 mins at 55°C
Discovery of a restriction enzyme, PaqCI, with a 7-bp recognition sequence
PaqCI® is a new Type IIS enzyme for Golden Gate Assembly. PaqCI offers the desired combination of a 7-bp recognition sequence and the generation of a 4-base overhang.
Learn how PaqCI enables 20+ fragment assembly in our NEB Inspired blog post