Improving the N-glycopeptide binding properties of human Fbs1

A novel plasmid display method was applied to eliminate bias against sialylated N-glycans. The resulting FbsI-GYR protein is now capable of unbiased binding and enrichment of N-glycopeptides from complex biological samples.

Plasmid display for selection of improved binding affinity of Fbs1

 
Chen, M., Shi, X., Duke, R.M., Ruse, C.I., Dai, N., Taron, C.H. and Samuelson, J.C (2017) An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat. Commun. PMID: 28534482