Protocol for cleavage with Thermostable OGG (NEB #M0464)
Note: Thermostable OGG will function at temperatures between 25-80°C, but functions optimally between 65-75°C.
Set up the following reaction:
|Components||20 µl reaction||Final conc.|
|Thermostable OGG||1 µl||
|8-oxoG-containing DNA||Up to 80 pmol||
|rCutSmart Buffer (10X)||2 µl||1X|
|H20||up to 20 µl|
Gently mix the reaction by pipetting up and down and microfuge briefly.
Incubate at 65-75°C for 1 hour.