Where can I find protocols for using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7770 & #E7775)?

Protocols for using NEB #E7770 and #E7775 with NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) or NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400 & #E7405) reagents can be found within the relevant kit’s manual. Please refer to the following details to identify which manual contains the protocol(s) relevant to library prep:

  • Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490).
  • Protocol for use with NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400, #E7405).
  • Protocol for use with FFPE RNA, NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400, #E7405).
  • Protocol for use with Purified mRNA or rRNA Depleted RNA.
  • Protocol for use with rRNA Depleted FFPE RNA.
  • Appendix
  • Troubleshooting Guide