Removal of gDNA from RNA Preparations Using DNase I-XT (NEB #M0570)

  1. Set up the following reaction on ice:
    RNA ~ 10μg RNA
    DNase I-XT Reaction Buffer (10X) 10μl (1X)
    DNase I-XT 1μl (2 units)
    Nuclease-free H2O to 100μl
  2. Incubate at 37°C for 15 minutes.
Note: When using RNA in downstream applications, column purification with NEB's Monarch RNA Cleanup Kits (NEB #T2030#T2040, #T2050), or cleanup with phenol/chloroform extraction is recommended.

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Related Products:
DNase I-XT