Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 RT-PCR Module, including cDNA synthesis and cDNA amplification?

Protocols for use of NEB #E7626 can be found within the product manual. The current manual includes VarSkip Short primers and NEBNext ARTIC primers. The earlier manual includes only NEBNext ARTIC primers.

Both manuals include:

  • cDNA Synthesis

  • Targeted cDNA Amplification

  • Cleanup Amplified cDNA 

Protocols.io also provides interactive versions of protocols for NEBNext ARTIC in the NEBNext ARTIC Protocols Collection, where you can discover and share optimizations with the research community.