Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 RT-PCR Module, including cDNA synthesis and cDNA amplification?
Protocols for use of NEB #E7626 can be found within the product manual. The current manual includes VarSkip Short primers and NEBNext ARTIC primers. The earlier manual includes only NEBNext ARTIC primers.
Both manuals include:
- cDNA Synthesis
- Targeted cDNA Amplification
- Cleanup Amplified cDNA
Protocols.io also provides interactive versions of protocols for NEBNext ARTIC in the NEBNext ARTIC Protocols Collection, where you can discover and share optimizations with the research community.