Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 RT-PCR Module, including cDNA synthesis and cDNA amplification?

Protocols for use of NEB #E7626 can be found within the product manual. The current manual includes VarSkip Short v2 primers and NEBNext ARTIC primers. The earlier manual includes the original VarSkip Short primers and NEBNext ARTIC primers.

Both manuals include:

  • cDNA Synthesis

  • Targeted cDNA Amplification

  • Cleanup Amplified cDNA 

Protocols.io also provides interactive versions of protocols for NEBNext ARTIC in the NEBNext ARTIC Protocols Collection, where you can discover and share optimizations with the research community.