Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit, including cDNA synthesis, cDNA amplification and library prep?

Protocols for use of NEB #E7658 can be found within the product manual, including:

  • cDNA Synthesis
  • Targeted cDNA Amplification 
  • Cleanup Amplified cDNA 
  • Fragmentation and End Prep 
  • Adaptor Ligation 
  • Cleanup of Adaptor-ligated DNA 
  • PCR Enrichment of Adaptor-ligated DNA 
  • Cleanup of PCR Reaction

Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.