Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit, including cDNA synthesis, cDNA amplification and library prep, and the Express protocol option with reduced cleanup steps?

Protocols for use of NEB #E7658 can be found within the product manual, including:

  • Express protocol and Standard protocol options
  • cDNA Synthesis
  • Targeted cDNA Amplification 
  • Cleanup Amplified cDNA (Standard protocol only)
  • Fragmentation and End Prep 
  • Adaptor Ligation 
  • Cleanup of Adaptor-ligated DNA (Standard protocol only)
  • PCR Enrichment of Adaptor-ligated DNA 
  • Cleanup of PCR Reaction

Protocols.io also provides interactive versions of the Standard protocol and the Express protocol, where you can discover and share optimizations with the research community.