Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit, including cDNA synthesis, cDNA amplification and library prep?
Protocols for use of NEB #E7658 can be found within the product manual, including:
- cDNA Synthesis
- Targeted cDNA Amplification
- Cleanup Amplified cDNA
- Fragmentation and End Prep
- Adaptor Ligation
- Cleanup of Adaptor-ligated DNA
- PCR Enrichment of Adaptor-ligated DNA
- Cleanup of PCR Reaction
Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.