Protocol for cleavage with McrBC (M0272)
1X NEBuffer 2, supplemented with 100 µg/ml BSA, 1 mM GTP. Incubate at 37°C.
- Set up the following reaction on ice.
COMPONENT 50 µl REACTION NEBuffer 2 (10X)
5 µl (1X)
1 µl (10 units)
dH20 up to 50 μl
- Incubate at 37°C for 1-2 hours.
- Inactivate at 65°C for 20 minutes.