Protocol for Quick-Load Purple 1kb Plus DNA Ladder (N0550)
- Load 5-10 µl (0.5-1 µg) of Quick-Load Purple 1kb Plus DNA Ladder per gel lane.
- The Loading Dye comes with the Product.
- Quick-Load Purple 1 kb Plus DNA Ladder is stable for at least 6 months at 25°C.
For long term storage, store at 4°C or -20°C.
If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
- Preparation of DNA
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
- Usage recommendation
We recommend loading 5-10 ul (0.5-1 ug) of Quick-Load Purple 1 kb Plus DNA Ladder per gel lane. The Quick-Load Purple 1 kb Plus DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.