Protocol for Quick-Load Purple 100 bp DNA Ladder (N0551)
- Load 10 µl (0.5 µg) Quick-Load Purple 100 bp DNA Ladder per gel lane.
- The Gel Loading Dye comes with the Product itself.
- Preparation of DNA
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
- Usage recommendation
We recommend loading 10 ul (0.5 ug) of Quick-Load® Purple 100 bp DNA Ladder per gel lane. The Quick-Load® Purple 100 bp DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
- Quick-Load® Purple 100 bp DNA ladder is stable for at least 6 months at 25°C.
For long term storage, store at 4°C or -20°C.
If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.