Protocol for Quick-Load 1 kb Plus DNA Ladder (NEB #N0469)

  1. Load 5–10 µl (0.5–1.0 µg) of Quick-Load 1 kb Plus DNA Ladder per gel lane.


  1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. In general, it is recommended to load 1µl per mm of gel lane.
  3. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  4. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
  5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.