Protocol for Product TriDye™ 1 kb Plus DNA Ladder (NEB #N3270)
- Load 5-10 μl (0.5-1.0 μg) of TriDye 1 kb Plus DNA ladder per gel lane.
- For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
- The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
- We recommend loading 5-10 μl (0.5-1.0 μg) of TriDye DNA ladder per gel lane.
- In general, it is recommended to load 1ul per mm of gel lane.
- TriDye DNA Ladder is stable for at least 6 months at 25°C.