Protocol for Nucleoside Digestion Mix (M0649)

  1. Set up the following reaction.

    Water up to 20 µl*
    Nucleoside Digestion Mix Reaction Buffer (10X)  2 µl
    DNA or RNA Substrate up to 1 µg
    Nucleoside Digestion Mix 1 µl

    *Reactions using 1 µl of Nucleoside Digestion Mix can be scaled up to 50 µl without any loss in activity.

  2. Incubate at 37°C for 1 hour.

  3. Sample is now ready for LC-MS analysis, no additional purification is required.