Home Protocols α1-2,4,6 Fucosidase O Typical Reaction Conditions

α1-2,4,6 Fucosidase O Typical Reaction Conditions

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:

1.    Combine 100 nM of oligosaccharide and H2O (if necessary) to make a 9 μl total reaction volume.
2.    Add 1 μl of 10X GlycoBuffer 1 to make a 10 μl total reaction volume.
3.    Add 1 μl of α1-2,4,6 Fucosidase O.
4.    Incubate at 37°C for 1 hour

Note: removal of α1-2 and α1-4 fucose residues may require more enzyme and longer incubation times.