Typical GluC Digest Reaction Conditions (P8100)
- Reconstitute Endoproteinase GluC in 500 µl H2O
4 µl H2O
5 µl protein substrate (1 µg)
10 µl 2X GluC Reaction Buffer
0.5 µl 100 ng/µl Endoproteinase GluC
- Incubate at 37°C for 16 hrs
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