Suggested Protocol for Loading the Unstained Protein Standard (P7704)

  1. Gently mix the Unstained Protein Standard, Broad Range.
  2. Load 3 μl directly onto a mini-gel.
  3. To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.
  4. Run gel using constant voltage, according to manufacturer’s specifications.
  5. Run until dye front reaches bottom of gel, but does not run off.
  6. Stain and de-stain gel with protein stain of choice, following manufacturer’s instructions (i.e. Coomassie Brilliant Blue, Simply Blue™ Safe Stain, or GelCode™ Blue Safe Stain (Thermo Fisher).