Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)
- Combine up to 50 μg of human IgG and H2O to a volume of 25 μl.
- Add 2.5 μl of 10X GlycoBuffer 2.
- Add 1 μl (500 units) of PNGase F Glycerol Free (NEB# P0709) and 1 µl (80 units) of IdeZ Protease (IgG-specific).
- Incubate 2 hours at 37°C.