Typical cDNA Synthesis Protocol

  1. Component Final Conc./AMT.
    10X Isothermal Amplification Buffer 1X
    dNTP Mix (10 mM) 0.5 mM
    Random Primer Mix 6 µM
    RNase Inhibitor, Murine
    20 units
    WarmStart RTx Reverse Transcriptase 0.25 µl
    Total Reaction Volume 25 µl

  2. Mix by vortexing.

  3. Incubate for 5 minutes at 25°C for annealing and 10 minutes at 55°C for synthesis. 

  4. Heat Inactivate WarmStart RTx Reverse Transcriptase by incubation at 80°C for 10 minutes.