Typical Reaction Conditions for β-N-Acetylglucosaminidase S (P0744)

  1. Combine 1 μg of glycoprotein or 100 nM of oligosaccharide and H2O (if necessary) to make a 9 μl total reaction volume.
  2. Add 1 μl of GlycoBuffer 1 (10X) to make a 10 μl total reaction volume.
  3. Add 1 μl β-N-Acetylglucosaminidase S.
  4. Incubate at 37°C for 1 hour.
  • The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1-2 μl for 1 μg of glycoprotein or 100 nM of oligosachride for one hour in a 10-25 μl reaction. If there is still undigested material, let the reaction go overnight.
  • The reaction can be scaled up linearly