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  • E. coli DNA Ligase Protocol (M0205)

    Set-up the reaction as follows:

    DNA up to 5 µg
    10X E. coli DNA Ligase Reaction Buffer 2 µl
    E. coli DNA Ligase 1 µl (10 units)
    H2O up to 20 µl

    Incubate at 16°C for 30 minutes.
    Heat inactivate by incubating at 65°C for 20 minutes