Typical reaction conditions (P0709)

Typical reaction conditions are as follows:
  1. Combine 1–20 μg of glycoprotein, 1 μl of 10X Glycoprotein Denaturing Buffer and H2O (if necessary) to make a 10 μl total reaction volume.
  2. Denature glycoprotein by heating reaction at 100°C for 10 minutes.
  3. Make a total reaction volume of 20 μl by adding 2 μl 10X G7 Reaction Buffer, 2 μl 10% NP40, H2O and 1–2 μl PNGase F (Glycerol Free), Recombinant.
  4. Incubate reaction at 37°C for 1 hour.