General protocol to release nucleic acids prior to capillary or gel electrophoresis using Proteinase K (P8107)
- For a 10-50 uL reaction, containing 40 ng (0.4 pmol) labeled probe and up to 0.5 ug of a DNA/RNA modifying enzyme, add 0.5-1 ul Proteinase K and incubate at room temperature 15 min.
- Proceed to analysis step. For gel electrophoresis, add appropriate loading dye.
- Please note, Proteinase K works in most biological buffers. See FAQ .