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  • Plating Protocol for NEB PCR Cloning Kit

    1. Mix cells thoroughly by flicking or inversion and spread 50 μl of the 1 ml outgrowth onto 37°C pre-warmed agar plates containing 100 μg/ml ampicillin. If a 15 minute ligation time was used, also plate 50 μl of a 1:10 dilution prepared with SOC. 

    2. Invert plate and incubate overnight at 37°C or for 24 hours at 30°C. Do not use room temperature growth as the slow growth rate will interfere with selection of constructs with inserts.