5 Minute Transformation (C2523)
A shortened transformation protocol resulting in approximately 10% efficiency compared to the standard protocol may be suitable for applications where a reduced total number of transformants is acceptable.
Follow the High Efficiency Transformation Protocol above with the following changes:
1. Steps 3 and 5 are reduced to 2 minutes.
2. Omit outgrowth (step 7) completely for ampicillin-resistant plasmids or reduce the outgrowth time for other selective media as appropriate.