For libraries with different size fragment inserts, refer to Table 1 for the
appropriate volume of beads to be added. The size selection protocol is
based on a starting volume of 100 μl. The protocol below is for libraries
with a 300–450 bp insert size.
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The NEB PCR Cloning Kit allows direct cloning from any amplification reaction, with no purification needed!
The Gibson Assembly™ Cloning Kit is a robust method for assembling 1-2 DNA fragments, with a cloning vector, and ensuring high transformation efficiencies - all in a little less than 2 hours.