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  • Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)

    1. Prepare a 20 μl reaction as follows:
      DNA    1 pmol of DNA ends*
      CutSmart Buffer (10X)    2 µl
      rSAP    1 unit
      H2O, purified    to 20 µl**

    2. Incubate at 37°C for 30 minutes.

    3. Stop reaction by heat-inactivation at 65°C for 5 minutes.
    *Note: 1 pmol of DNA ends is about 1 µg of a 3 kb plasmid.

    **Scale larger reaction volumes proportionally.