Ethanol Precipitation - NEBNext Microbiome DNA Enrichment Kit (E2612)

  1. Add 2.5 volumes of ethanol, incubate for 10 minutes on ice, then centrifuge the sample at 13,000 rpm (16,000 rcf). Remove the residual ethanol, allow the pellet to air dry, and then resuspend the pellet in a small quantity (50 μl) of TE buffer.

  2. If the ethanol pellet contained any residual beads, the resuspended sample can be placed on the magnetic rack 5 minutes to concentrate the beads on the inner wall of the tube, and the supernatant removed to a fresh microcentrifuge tube.

  3. The sample can now be used for NGS library construction or other downstream analysis.
NGS Library Construction

Enriched samples are compatible with NEBNext DNA Library Prep reagents for the following platforms: