Collect Enriched Microbial DNA - NEBNext Microbiome DNA Enrichment Kit (E2612)

  1. After incubating the DNA and MBD2-Fc-bound magnetic beads, briefly spin the tube and place on the magnetic rack for 5 minutes until the beads have collected to the wall of the tube and the solution is clear. 

  2. Carefully remove the supernatant with a pipette, without disturbing the beads, and save it in a clean microcentrifuge tube. This supernatant contains the target microbial DNA. Store this sample at –20˚C or proceed directly to purification.

  3. Purify the samples by either Ampure bead clean-up or ethanol precipitation.

  4. To elute captured host DNA, see here.