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  • Sample preparation using provided RNA Loading Dye (2X) and denaturing conditions (N0364)

    Protocol

    This method utilizes the RNA Loading Dye, (2X) provided and samples should be run on a denaturing polyacrylamide-urea gel prepared with 1X TBE.

    1. Combine on ice:
      Low Range ssRNA Ladder (500 μg/ml)   0.5 μl (0.25 μg)
      H2O (RNase-free)   4.5 μl
      RNA Loading Dye, (2X)   5 μl
      ------------------------------------------------------------------------------
      Total Volume   10 μl

    2. Heat at 65°C for 5 minutes, chill on ice, load entire sample on gel.

    3. Run gel according to manufacturer's conditions. All six bands of the marker can be resolved on a 6% gel. 0.5 μg/ml ethidium bromide will effectively stain the bands after electrophoresis.