Home Protocols Sample preparation using provided RNA Loading Dye (2X) and denaturing conditions (N0364)

Sample preparation using provided RNA Loading Dye (2X) and denaturing conditions (N0364)

Protocol

This method utilizes the RNA Loading Dye, (2X) provided and samples should be run on a denaturing polyacrylamide-urea gel prepared with 1X TBE.

  1. Combine on ice:
    Low Range ssRNA Ladder (500 μg/ml)   0.5 μl (0.25 μg)
    H2O (RNase-free)   4.5 μl
    RNA Loading Dye, (2X)   5 μl
    ------------------------------------------------------------------------------
    Total Volume   10 μl

  2. Heat at 65°C for 5 minutes, chill on ice, load entire sample on gel.

  3. Run gel according to manufacturer's conditions. All six bands of the marker can be resolved on a 6% gel. 0.5 μg/ml ethidium bromide will effectively stain the bands after electrophoresis.