Optimal incubation times
and enzyme concentrations must be determined
empirically for a particular substrate. Typical
reaction conditions are as follows:
- Combine 100 µg of native IgG, 1 µl of 10X GlycoBuffer 1 and H20 (if necessary) to make a
10 µl total reaction volume.
- Add 1 µl Endo S.
- Incubate reaction at 37°C for 1 hour.