Perform Second Strand cDNA Synthesis (E7530)


  1. Add the following reagents to the First Strand Synthesis reaction (20 μl):

    Nuclease-free water   48 μl
    Second Strand Synthesis Reaction Buffer (10X)   8 μl
    Second Strand Synthesis Enzyme Mix   4 μl
    Total volume   80 μl

  2. Mix thoroughly by gentle pipetting.

  3. Incubate in a thermal cycler for 1 hour at 16°C, with heated lid set at ≤ 40°C.