NEBNext End Prep (E7370)

Starting Material: 5 ng–1 μg fragmented DNA.

Protocol

  1. Mix the following components in a sterile nuclease-free tube: 
    End Prep Enzyme Mix   3.0 μl
    End Repair Reaction Buffer (10X)   6.5 μl
    Fragmented DNA   55.5 μl
    --------------------------------------------------------------------
    Total volume   65 μl 

  2. Mix by pipetting followed by a quick spin to collect all liquid from the sides of the tube.

  3. Place in a thermocycler, with the heated lid on, and run the follwing program:
    30 minutes @ 20°C
    30 minutes @ 65°C
    Hold at 4°C