• My NEB
  • Print
  • PDF
  • NEBNext End Prep (E7370)

    Starting Material: 5 ng–1 μg fragmented DNA.

    Protocol

    1. Mix the following components in a sterile nuclease-free tube: 
      End Prep Enzyme Mix   3.0 μl
      End Repair Reaction Buffer (10X)   6.5 μl
      Fragmented DNA   55.5 μl
      --------------------------------------------------------------------
      Total volume   65 μl 

    2. Mix by pipetting followed by a quick spin to collect all liquid from the sides of the tube.

    3. Place in a thermocycler, with the heated lid on, and run the follwing program:
      30 minutes @ 20°C
      30 minutes @ 65°C
      Hold at 4°C