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  • Perform End Repair/dA-tail of cDNA Library (E7420)

    Protocol

    1. To the purified double-stranded cDNA (55.5 μl), add the following components:
      NEBNext End Repair Reaction Buffer (10X)   6.5 μl
      NEBNext End Prep Enzyme Mix   3 μl
      ---------------------------------------------------------------------------
      Total volume   65 μl

    2. Incubate the sample in a thermal cycler as follows:
      30 minutes at 20°C
      30 minutes at 65°C
      Hold at 4°C

    3. Proceed immediately to Adaptor Ligation.