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  • Sample Preparation Methods for Single Cell WGA Kit

    Protocol

    1. Cell Sample (5 μl)
      1. Wash or dilute cells with 1X PBS buffer, according to the recommendations in the Cell Specifications section of the manual.
      2. If collecting cells by flow sorting: Collect a single cell into 5 μl of Cell Extraction Buffer in a PCR tube or well. If collecting cells by micromanipulation or dilution: Transfer a single cell in minimal 1X PBS volume (< 2.5 μl) to a PCR tube or well containing an appropriate volume of Cell Extraction Buffer to achieve a total cell sample volume of 5 μl.
      3. Immediately freeze and store cells at -80°C or proceed directly to the Pre-Amplification Protocol.

    2. Control DNA Sample (5 μl)
      1. Prepare a 1 ng/μl purified DNA solution in a PCR tube or well by diluting a control DNA stock with 5 mM Tris-HCl (pH 8.0).
      2. Vortex the 1 ng/μl DNA solution for 30 seconds.
      3. Add 3 μl of the 1 ng/μl DNA solution to 197 μl of 5 mM Tris-HCl (pH 8.0) to prepare a 15 pg/μl DNA sample.
      4. Vortex the 15 pg/μl DNA solution for 30 seconds.
      5. Add 1 μl of the 15 pg/μl DNA solution to 4 μl of Cell Extraction Buffer in a PCR tube or well.