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  • Pre-Amplification Protocol for PicoPLEX WGA Kit

    Protocol

    1. Combine Extraction Cocktail components and mix well:
      EXTRACTION COCKTAIL VOLUME PER 5 SAMPLES
      Extraction Enzyme Dilution Buffer 24 μl
      Cell Extraction Enzyme 1 μl
      Total volume 25 μl
    2. Add 5 μl of freshly-prepared Extraction Cocktail to each 5 μl cell sample or control DNA sample prepared by the sample preparation methods.

    3. Incubate sample in a thermal cycler as follows:
      CYCLES
      TEMP
      TIME
      1
      75°C
      10 minutes
      95°C
      4 minutes
      12°C
      Hold
    4. Combine Pre-Amp Cocktail components and mix well:
      PRE-AMP COCKTAIL VOLUME PER 5 SAMPLES
      Pre-Amp Reaction Mix 24 μl
      Pre-Amp Enzyme 1 μl
      Total volume 25 μl
    5. Add 5 μl of Pre-Amp Cocktail to each cell or control sample.

    6. Incubate sample according to the thermal cycler program below:
      CYCLES TEMP
      TIME
      1 95°C
      2 minutes
      12 95°C 15 seconds
      15°C 50 seconds
      25°C 40 seconds
      35°C 30 seconds
      65°C 40 seconds
      75°C 40 seconds
      1 4°C Hold
    7. Briefly centrifuge sample and place Pre-Amp incubation product on ice.