Preparing and Loading Protein Ladders
- Thaw the Prestained Protein Ladder at room temperature. Vortex gently to ensure the solution is homogeneous.
- Transfer the desired amount of the Prestained Protein Ladder to a separate tube. For blotting: use 5 µl for mini-gels and 10 µl for full length gels. For visualizing during electrophoresis: use 10-15 µl for mini-gels and 20-30 µl for full length gels.
- Heat the Prestained Protein Ladder at 95-100°C for 3-5 minutes. If this ladder has already been boiled for storage (see storage note), no further heating is necessary.
- Load directly onto an SDS-PAGE gel and electrophorese.