Solutions Required for Western Analysis (E8023)

Introduction   

10X TBS (per liter):
24.2g Tris base
80g NaCl
Milli-Q™ water
pH 7.6 

1X TBS/T (per liter):
100 ml 10X TBS
1 ml Tween-20
800 ml MilliQ water 

Blocking Buffer (in 1X TBS/T):
2.5% BSA
2.5% non-fat milk 

Primary Antibody Solution:
1:2000 dilution of Anti-GLuc Antibody in Blocking Buffer 

HRP-conjugated Secondary Antibody Solution:
1:2000 dilution of Anti-rabbit IgG in Blocking Buffer